{"id":"2c8e7d79-0665-50d0-a98f-0adff78dfbd8","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-pmca1-extrusion","predicate":"transports","statement":"Human PMCA1 is an ATP-driven plasma-membrane calcium extrusion pump characterized structurally with neuroplastin.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fddf8922-55e3-5650-ae7a-1b48fff85c3a","mechanism_event_label":"PMCA1 uses ATP to clear calcium from the cytosol.","subject":{"id":"dd68a62e-78df-5944-a783-48b9818aeed1","slug":"atp2b1","display_name":"Plasma membrane calcium ATPase 1 / ATP2B1","entity_type_key":"protein"},"object":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"fddf8922-55e3-5650-ae7a-1b48fff85c3a","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-pmca1-extrusion-event","event_type":"transport","label":"PMCA1 uses ATP to clear calcium from the cytosol.","description":"Human PMCA1 is an ATP-driven plasma-membrane calcium extrusion pump characterized structurally with neuroplastin.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"dd68a62e-78df-5944-a783-48b9818aeed1","slug":"atp2b1","display_name":"Plasma membrane calcium ATPase 1 / ATP2B1","entity_type_key":"protein"},"role":"pump","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"energy_substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c4fa0bb7-fb71-5a56-8e5f-61a7dadc682c","slug":"nptn","display_name":"Neuroplastin / NPTN","entity_type_key":"protein"},"role":"auxiliary_subunit","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f0b6bcb6-5d41-5d9d-af9f-6af1a94d5c5a","slug":"plasma-membrane","display_name":"Plasma membrane","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":4,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Plasma membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human PMCA1-neuroplastin complex; cryo-EM and ATPase assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This study combines structure and ATPase assays; it does not quantify whole-tissue extrusion flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PMCA1 uses ATP to clear calcium from the cytosol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-gong2018] Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin (2018). https://pubmed.ncbi.nlm.nih.gov/30190470/ DOI: 10.1038/s41467-018-06075-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"transport","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant pump","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"lowers","comparator":null,"unit":null,"notes":"Recorded as an ATP-driven plasma-membrane calcium extrusion pump.","entity":null},{"dimension":"transport_or_reaction_direction","value_text":"Cytosol to extracellular fluid","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_pool","value_text":"cytosolic calcium","comparator":null,"unit":null,"notes":"Recorded as an ATP-driven plasma-membrane calcium extrusion pump.","entity":null}],"evidence":[{"id":"b6919b32-ff69-5ee0-8cb8-e2706fb7de53","evidence_kind":"source_excerpt","locator":"Lines 585-597","start_line":585,"end_line":597,"excerpt":"### ca-pmca1-extrusion\nHuman PMCA1 is an ATP-driven plasma-membrane calcium extrusion pump characterized structurally with neuroplastin.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: PMCA1 uses ATP to clear calcium from the cytosol.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant pump\nexperimental_model: Purified human PMCA1-neuroplastin complex; cryo-EM and ATPase assays\nlimitations: This study combines structure and ATPase assays; it does not quantify whole-tissue extrusion flux.\nresearch_relationship_category: transport\ntransport_or_reaction_direction: Cytosol to extracellular fluid\ncompartment_description: Plasma membrane\n[ca-gong2018] Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin (2018). https://pubmed.ncbi.nlm.nih.gov/30190470/ DOI: 10.1038/s41467-018-06075-7","model_system":"Purified human PMCA1-neuroplastin complex; cryo-EM and ATPase assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ca-gong2018] Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin (2018). https://pubmed.ncbi.nlm.nih.gov/30190470/ DOI: 10.1038/s41467-018-06075-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}