{"id":"2c5e78ac-03cf-5265-bbf8-9deb017c1957","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-aars-lactylation","predicate":"catalyzes_lactate_dependent","statement":"Human AARS1 used lactate and ATP for protein lactylation; the study identified YAP K90 and TEAD1 K108 modification and activation in gastric cancer models.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c3ab6478-ed48-5592-bbd5-ccf3acfe3184","mechanism_event_label":"The alanine-handling enzyme also has a separate lactate-driven signaling activity.","subject":{"id":"cc0bb2ab-8cf9-54a7-af05-3681ef835010","slug":"aars1","display_name":"Human cytosolic alanyl-tRNA synthetase / AARS1","entity_type_key":"protein"},"object":{"id":"32bd14a7-f85c-50bf-834c-cb70f084830c","slug":"human-aars1-yap-tead-lactylation","display_name":"AARS1-dependent YAP/TEAD lactylation in human gastric cancer models","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c3ab6478-ed48-5592-bbd5-ccf3acfe3184","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-aars-lactylation-event","event_type":"observed_relationship","label":"The alanine-handling enzyme also has a separate lactate-driven signaling activity.","description":"Human AARS1 used lactate and ATP for protein lactylation; the study identified YAP K90 and TEAD1 K108 modification and activation in gastric cancer models.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cc0bb2ab-8cf9-54a7-af05-3681ef835010","slug":"aars1","display_name":"Human cytosolic alanyl-tRNA synthetase / AARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32bd14a7-f85c-50bf-834c-cb70f084830c","slug":"human-aars1-yap-tead-lactylation","display_name":"AARS1-dependent YAP/TEAD lactylation in human gastric cancer models","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5ebd9a7c-b151-5b70-b70d-7d33067d4ebf","slug":"lactate","display_name":"L-Lactate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"442361fa-769a-553d-a37f-41a3f608a0a4","slug":"yap1","display_name":"Human YAP1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"09ee2532-353e-58f5-b4dd-4f69c0cd3aa4","slug":"tead1","display_name":"Human TEAD1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant enzyme and human gastric cancer cell experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is an enzyme connection, not proof that adding L-alanine promotes or inhibits lactylation. Beta-alanine inhibitor findings must not be transferred to L-alanine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The alanine-handling enzyme also has a separate lactate-driven signaling activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The alanyl-tRNA synthetase AARS1 moonlights as a lactyltransferase to promote YAP signaling in gastric cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38512451/ · DOI 10.1172/JCI174587","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2580d24a-1fc2-5031-938e-83a04f351615","evidence_kind":"source_excerpt","locator":"Lines 72-78","start_line":72,"end_line":78,"excerpt":"## alanine-aars-lactylation\nThe alanine-handling enzyme also has a separate lactate-driven signaling activity.\nHuman AARS1 used lactate and ATP for protein lactylation; the study identified YAP K90 and TEAD1 K108 modification and activation in gastric cancer models.\nModel: Recombinant enzyme and human gastric cancer cell experiments.\nLimitations: This is an enzyme connection, not proof that adding L-alanine promotes or inhibits lactylation. Beta-alanine inhibitor findings must not be transferred to L-alanine.\nEvidence access: Primary full text\nThe alanyl-tRNA synthetase AARS1 moonlights as a lactyltransferase to promote YAP signaling in gastric cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38512451/ · DOI 10.1172/JCI174587","model_system":"Recombinant enzyme and human gastric cancer cell experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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