{"id":"2c5bfc17-79fe-5114-9e3a-48ce8bd450ae","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-slc25a48-import","predicate":"mediates","statement":"Human-cell experiments identified SLC25A48 as necessary for efficient mitochondrial choline import.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a69985bf-5846-528c-9303-6248e22988f9","mechanism_event_label":"Choline must cross another membrane to reach its mitochondrial metabolic route.","subject":{"id":"b734847a-880d-5f17-9015-1c93037b9cb4","slug":"slc25a48","display_name":"Human mitochondrial choline carrier SLC25A48","entity_type_key":"protein"},"object":{"id":"67a796d5-927f-5a87-b620-4235b89d3ff7","slug":"human-mitochondrial-choline-import","display_name":"Human mitochondrial choline import","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a69985bf-5846-528c-9303-6248e22988f9","stable_key":"27e0c1cf-7726-5164-8b15-27a631584cd0:choline-slc25a48-import-event","event_type":"biochemical_relationship","label":"Choline must cross another membrane to reach its mitochondrial metabolic route.","description":"Human-cell experiments identified SLC25A48 as necessary for efficient mitochondrial choline import.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8e1d19fd-ad66-575d-81c4-9122f78915e5","slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"},"role":"imported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b734847a-880d-5f17-9015-1c93037b9cb4","slug":"slc25a48","display_name":"Human mitochondrial choline carrier SLC25A48","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"67a796d5-927f-5a87-b620-4235b89d3ff7","slug":"human-mitochondrial-choline-import","display_name":"Human mitochondrial choline import","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/choline-research/39111307.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\", \"start_char\": 0, \"end_char\": 1201, \"text_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Germline mouse knockout; human-cell SLC25A48 loss; labeled choline","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport, energy and one-carbon outcomes are model-specific; normal intake cannot be assumed to correct a carrier defect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Choline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"choline","display_name":"Choline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Choline must cross another membrane to reach its mitochondrial metabolic route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. (2024). https://pubmed.ncbi.nlm.nih.gov/39111307/ DOI: 10.1016/j.cmet.2024.07.010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brown adipose tissue and human HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f5c756af-24c9-55b5-bad2-df77190ccbcd","evidence_kind":"source_excerpt","locator":"Lines 477-488","start_line":477,"end_line":488,"excerpt":"### choline-slc25a48-import\nHuman-cell experiments identified SLC25A48 as necessary for efficient mitochondrial choline import.\nCondition category: normal\nnutrient_topic: Choline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Choline must cross another membrane to reach its mitochondrial metabolic route.\norganism: Human HEK293T cells\ntissue_or_cell_type: Brown adipose tissue and human HEK293T cells\nexperimental_model: Slc25a48 knockout, rescue, mitochondrial assays and isotope tracing\nlimitations: Transport, energy and one-carbon outcomes are model-specific; normal intake cannot be assumed to correct a carrier defect.\nexposure: Germline mouse knockout; human-cell SLC25A48 loss; labeled choline\nevidence_span: {\"source_cache\": \"artifacts/choline-research/39111307.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\", \"start_char\": 0, \"end_char\": 1201, \"text_sha256\": \"c0a9377732782bdaa55105cae0a0446afcfb1225e686d2b047a7fdf1ef67535f\"}\n[choline-p39111307] SLC25A48 controls mitochondrial choline import and metabolism. 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