{"id":"2c51066a-023b-5dbf-b1b7-835530b3834e","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-phospho1-cofactor","predicate":"supports_catalytic_activity","statement":"Recombinant human PHOSPHO1 showed strong Mg dependence when hydrolyzing phosphoethanolamine and phosphocholine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8d6c6026-322f-598f-978c-dd3c78692aa7","mechanism_event_label":"Magnesium helps a phosphate-releasing enzyme already connected to calcium mineralization.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"bb7ed19e-2f65-5951-8963-40d6b3249b71","slug":"phospho1","display_name":"Phosphoethanolamine/phosphocholine phosphatase PHOSPHO1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8d6c6026-322f-598f-978c-dd3c78692aa7","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-phospho1-cofactor-event","event_type":"biochemical_relationship","label":"Magnesium helps a phosphate-releasing enzyme already connected to calcium mineralization.","description":"Recombinant human PHOSPHO1 showed strong Mg dependence when hydrolyzing phosphoethanolamine and phosphocholine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cd538be5-1596-5a39-97e4-d3c12137e6be","slug":"phosphoethanolamine","display_name":"Phosphoethanolamine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2159176b-2e94-5403-98e9-97e966b38b81","slug":"phosphocholine","display_name":"Phosphocholine","entity_type_key":"small_molecule"},"role":"alternative_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; 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substrate Km values are not Mg deficiency thresholds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Magnesium helps a phosphate-releasing enzyme already connected to calcium mineralization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mg-roberts2004] Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities (2004). https://pubmed.ncbi.nlm.nih.gov/15175005/ DOI: 10.1042/bj20040511","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant human PHOSPHO1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"54d059e5-2666-5889-8ffe-3b8e3b21b08c","evidence_kind":"source_excerpt","locator":"Lines 1455-1465","start_line":1455,"end_line":1465,"excerpt":"### mg-phospho1-cofactor\nRecombinant human PHOSPHO1 showed strong Mg dependence when hydrolyzing phosphoethanolamine and phosphocholine.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Magnesium helps a phosphate-releasing enzyme already connected to calcium mineralization.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant human PHOSPHO1\nexperimental_model: Purified recombinant phosphatase assays.\nlimitations: Enzyme evidence does not measure the effect of oral Mg on bone; substrate Km values are not Mg deficiency thresholds.\ncross_nutrient: Magnesium -> PHOSPHO1 -> phosphate production; joins the existing calcium PHOSPHO1 records.\n[mg-roberts2004] Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities (2004). https://pubmed.ncbi.nlm.nih.gov/15175005/ DOI: 10.1042/bj20040511","model_system":"Purified recombinant phosphatase assays.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mg-roberts2004] Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities (2004). https://pubmed.ncbi.nlm.nih.gov/15175005/ DOI: 10.1042/bj20040511","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}