{"id":"2c3af7fd-5ba9-50cb-bf2f-4f30770b1373","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-fsp1-carboxylation","predicate":"supports_warfarin_resistant","statement":"A CRISPR screen independently identified FSP1 as the warfarin-resistant vitamin K reductase; FSP1 inhibition impaired vitamin K-dependent carboxylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"751bea6e-c309-53ac-adc5-a286dc6e191d","mechanism_event_label":"The antioxidant enzyme also helps supply cofactor for protein modification.","subject":{"id":"6c60c705-b3dc-548d-a2ad-e9f75249a642","slug":"aifm2","display_name":"FSP1 / AIFM2","entity_type_key":"protein"},"object":{"id":"de0220e7-19ea-5b7e-8521-57abbfe8e945","slug":"vitamin-k-dependent-carboxylation","display_name":"Vitamin K-dependent protein gamma-carboxylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"751bea6e-c309-53ac-adc5-a286dc6e191d","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-fsp1-carboxylation-event","event_type":"biochemical_relationship","label":"The antioxidant enzyme also helps supply cofactor for protein modification.","description":"A CRISPR screen independently identified FSP1 as the warfarin-resistant vitamin K reductase; FSP1 inhibition impaired vitamin K-dependent carboxylation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ee94b256-deeb-5795-8b6e-6e388576d464","slug":"warfarin","display_name":"Warfarin","entity_type_key":"small_molecule"},"role":"tested_inhibitor_context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8889bae4-1223-5f80-b651-0c42190322a9","slug":"ggcx","display_name":"Gamma-glutamyl carboxylase / GGCX","entity_type_key":"protein"},"role":"downstream_enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6c60c705-b3dc-548d-a2ad-e9f75249a642","slug":"aifm2","display_name":"FSP1 / AIFM2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"de0220e7-19ea-5b7e-8521-57abbfe8e945","slug":"vitamin-k-dependent-carboxylation","display_name":"Vitamin K-dependent protein gamma-carboxylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/k2-research/36788244.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5fda09197d386351a1b1d261c9b385db36e02eab0c238bf18599d7086b501f93\", \"start_char\": 0, \"end_char\": 1359, \"text_sha256\": \"5fda09197d386351a1b1d261c9b385db36e02eab0c238bf18599d7086b501f93\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Genome-wide CRISPR screen and reporter biochemistry","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"FSP1 knockout/inhibition and DHODH comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Supports cycle biochemistry; a different ferroptosis defense enzyme need not share the same vitamin K function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"k2","display_name":"Vitamin K2 / menaquinone family","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human vitamin K-dependent reporter cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The antioxidant enzyme also helps supply cofactor for protein modification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[k2-p36788244] A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase. (2023). https://pubmed.ncbi.nlm.nih.gov/36788244/ DOI: 10.1038/s41467-023-36446-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Warfarin-resistant quinone reduction","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9170849e-e070-5df4-ba8c-fae8acc5e0c8","evidence_kind":"source_excerpt","locator":"Lines 825-836","start_line":825,"end_line":836,"excerpt":"### k2-fsp1-carboxylation\nA CRISPR screen independently identified FSP1 as the warfarin-resistant vitamin K reductase; FSP1 inhibition impaired vitamin K-dependent carboxylation.\nCondition category: normal\nnutrient_topic: Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The antioxidant enzyme also helps supply cofactor for protein modification.\norganism: Human vitamin K-dependent reporter cells\ntissue_or_cell_type: Warfarin-resistant quinone reduction\nexperimental_model: Genome-wide CRISPR screen and reporter biochemistry\nlimitations: Supports cycle biochemistry; a different ferroptosis defense enzyme need not share the same vitamin K function.\nexposure: FSP1 knockout/inhibition and DHODH comparison\nevidence_span: {\"source_cache\": \"artifacts/k2-research/36788244.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5fda09197d386351a1b1d261c9b385db36e02eab0c238bf18599d7086b501f93\", \"start_char\": 0, \"end_char\": 1359, \"text_sha256\": \"5fda09197d386351a1b1d261c9b385db36e02eab0c238bf18599d7086b501f93\"}\n[k2-p36788244] A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase. (2023). https://pubmed.ncbi.nlm.nih.gov/36788244/ DOI: 10.1038/s41467-023-36446-8","model_system":"Genome-wide CRISPR screen and reporter biochemistry","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [k2-p36788244] A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase. (2023). https://pubmed.ncbi.nlm.nih.gov/36788244/ DOI: 10.1038/s41467-023-36446-8","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dfeae572-af3d-5042-a10e-e67596f9ab28","stable_key":"import-4fef8d72-f107-5eb5-b2fb-483ca85465e0","title":"Vitamin K2: menaquinone forms, carboxylation, recycling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"dc31aff21110d09b025c177e03b4c5ca8349d52bf5f962285fdbfcf660c0e4f6","revision_id":"850887db-34f6-55aa-9d3e-b8fd3a4a5de3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}