{"id":"2c2dedcb-a791-5c0d-b0ed-1f1133b0f5c8","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:trpm6-trpm7-coassembly","predicate":"assembles-with","statement":"TRPM6 coassembled with TRPM7 to form functional surface channel complexes in the tested expression systems.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a03eaebb-029f-5c4d-b5a1-5c1449dad7ab","mechanism_event_label":"A TRPM7 partner enabled TRPM6-containing channels to reach and function at the cell surface.","subject":{"id":"877a2fb0-067c-51ca-afea-f63ff16affc8","slug":"trpm6","display_name":"TRPM6","entity_type_key":"protein"},"object":{"id":"16ade5d1-e269-5904-9ab1-b285509b7aef","slug":"trpm7","display_name":"TRPM7","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a03eaebb-029f-5c4d-b5a1-5c1449dad7ab","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:trpm6-trpm7-coassembly-event","event_type":"biochemical_relationship","label":"A TRPM7 partner enabled TRPM6-containing channels to reach and function at the cell surface.","description":"TRPM6 coassembled with TRPM7 to form functional surface channel complexes in the tested expression systems.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"80ac07f5-bdf8-5714-8b8c-c687f59bb519","slug":"trpm6-trpm7-complex","display_name":"TRPM6/TRPM7 heteromeric channel complex","entity_type_key":"protein_complex"},"role":"assembled-complex","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"permeant-ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"877a2fb0-067c-51ca-afea-f63ff16affc8","slug":"trpm6","display_name":"TRPM6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"16ade5d1-e269-5904-9ab1-b285509b7aef","slug":"trpm7","display_name":"TRPM7","entity_type_key":"protein"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence-system","value_text":"HEK293 and Xenopus heterologous coexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"HEK293 and Xenopus heterologous coexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"TRPM6 isoforms and cellular background affect results; not proof every native complex has identical composition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human proteins; human-derived and Xenopus cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A TRPM7 partner enabled TRPM6-containing channels to reach and function at the cell surface.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chubanov-2004-trpm6-trpm7] Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia. (2004). https://pubmed.ncbi.nlm.nih.gov/14976260/ DOI: 10.1073/pnas.0305252101","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Expression systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Expression systems","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"50781f83-a79d-54a4-9a52-47ae89aac147","evidence_kind":"source_excerpt","locator":"Lines 883-894","start_line":883,"end_line":894,"excerpt":"### trpm6-trpm7-coassembly\nTRPM6 coassembled with TRPM7 to form functional surface channel complexes in the tested expression systems.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A TRPM7 partner enabled TRPM6-containing channels to reach and function at the cell surface.\norganism: Human proteins; human-derived and Xenopus cells\ntissue_or_cell_type: Expression systems\nexperimental_model: HEK293 and Xenopus heterologous coexpression\nlimitations: TRPM6 isoforms and cellular background affect results; not proof every native complex has identical composition.\nevidence-system: HEK293 and Xenopus heterologous coexpression\ntissue: Expression systems\n[chubanov-2004-trpm6-trpm7] Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia. (2004). https://pubmed.ncbi.nlm.nih.gov/14976260/ DOI: 10.1073/pnas.0305252101","model_system":"HEK293 and Xenopus heterologous coexpression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chubanov-2004-trpm6-trpm7] Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia. (2004). https://pubmed.ncbi.nlm.nih.gov/14976260/ DOI: 10.1073/pnas.0305252101","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}