{"id":"2c2bd7e0-bd0c-59ad-82c4-2cdddaeac676","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-sheep-ttp-vps35","predicate":"coimmunoprecipitates_with","statement":"Co-immunoprecipitation/Western blot experiments identified an association of sheep alpha-TTP with Vps35 in a liver/primary-hepatocyte study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"ac7c1086-b949-578d-8507-ee582bcce372","mechanism_event_label":"The sheep study linked this trafficking protein with alpha-TTP.","subject":{"id":"d1cc5e35-dbc0-5e98-93ed-2acdf7592f26","slug":"sheep-ttpa","display_name":"Sheep alpha-tocopherol transfer protein","entity_type_key":"protein"},"object":{"id":"3d04e6ec-21e3-5995-b19d-87549cb70f82","slug":"sheep-vps35","display_name":"Sheep VPS35 protein","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ac7c1086-b949-578d-8507-ee582bcce372","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-sheep-ttp-vps35-event","event_type":"biochemical_relationship","label":"The sheep study linked this trafficking protein with alpha-TTP.","description":"Co-immunoprecipitation/Western blot experiments identified an association of sheep alpha-TTP with Vps35 in a liver/primary-hepatocyte study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d1cc5e35-dbc0-5e98-93ed-2acdf7592f26","slug":"sheep-ttpa","display_name":"Sheep alpha-tocopherol transfer protein","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3d04e6ec-21e3-5995-b19d-87549cb70f82","slug":"sheep-vps35","display_name":"Sheep VPS35 protein","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Co-IP/Western blot validation in sheep liver/hepatocyte study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Endogenous interaction profiling and validation; exact pull-down exposures not assigned from accessible sections.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Association is not proof of direct binary binding or a defined stable complex; no human nutritional outcome is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Ovis aries","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The sheep study linked this trafficking protein with alpha-TTP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[jian2025] Rab8a and Vps35 influence intracellular transport of vitamin E via α-Tocopherol transport protein in hepatocytes. (2025). https://pubmed.ncbi.nlm.nih.gov/40258550/ DOI: 10.1016/j.ijbiomac.2025.143021","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver and newborn male Australian White sheep primary hepatocytes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"76385fd5-8f35-5514-baf2-3b26f8f2efa0","evidence_kind":"source_excerpt","locator":"Lines 389-400","start_line":389,"end_line":400,"excerpt":"### ve-transport-sheep-ttp-vps35\nCo-immunoprecipitation/Western blot experiments identified an association of sheep alpha-TTP with Vps35 in a liver/primary-hepatocyte study.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The sheep study linked this trafficking protein with alpha-TTP.\norganism: Ovis aries\ntissue_or_cell_type: Liver and newborn male Australian White sheep primary hepatocytes\nexperimental_model: Co-IP/Western blot validation in sheep liver/hepatocyte study\nlimitations: Association is not proof of direct binary binding or a defined stable complex; no human nutritional outcome is inferred.\nexposure: Endogenous interaction profiling and validation; exact pull-down exposures not assigned from accessible sections.\ncross_nutrient: false\n[jian2025] Rab8a and Vps35 influence intracellular transport of vitamin E via α-Tocopherol transport protein in hepatocytes. (2025). https://pubmed.ncbi.nlm.nih.gov/40258550/ DOI: 10.1016/j.ijbiomac.2025.143021","model_system":"Co-IP/Western blot validation in sheep liver/hepatocyte study","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [jian2025] Rab8a and Vps35 influence intracellular transport of vitamin E via α-Tocopherol transport protein in hepatocytes. (2025). https://pubmed.ncbi.nlm.nih.gov/40258550/ DOI: 10.1016/j.ijbiomac.2025.143021","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3ac41dba-4727-5116-a556-ac3bbb0dc41c","stable_key":"import-e0ea2d6a-7429-5e9f-b774-41e5e3288da3","title":"Vitamin E: transport, membrane protection and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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