{"id":"2c2a3694-4ea3-5c0c-b11b-229074299078","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-elongation-quality-control","predicate":"binds_weakly_to","statement":"The glutamate-mischarged tRNA(Gln) intermediate bound mitochondrial EF-Tu weakly, supporting exclusion from translation before correction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"abf4de41-e428-5200-ac67-218f5d6a44df","mechanism_event_label":"Quality control reduces entry of the unfinished intermediate into protein synthesis.","subject":{"id":"d7bb28eb-0aef-5be7-b455-c801b311ca61","slug":"human-mt-glu-trna-gln","display_name":"Human mitochondrial glutamyl-tRNA(Gln)","entity_type_key":"rna"},"object":{"id":"fc051549-e223-5385-80b3-adf130936359","slug":"tufm","display_name":"Human mitochondrial elongation factor Tu / TUFM","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"abf4de41-e428-5200-ac67-218f5d6a44df","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-elongation-quality-control-event","event_type":"observed_relationship","label":"Quality control reduces entry of the unfinished intermediate into protein synthesis.","description":"The glutamate-mischarged tRNA(Gln) intermediate bound mitochondrial EF-Tu weakly, supporting exclusion from translation before correction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d7bb28eb-0aef-5be7-b455-c801b311ca61","slug":"human-mt-glu-trna-gln","display_name":"Human mitochondrial glutamyl-tRNA(Gln)","entity_type_key":"rna"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fc051549-e223-5385-80b3-adf130936359","slug":"tufm","display_name":"Human mitochondrial elongation factor Tu / TUFM","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human mitochondrial tRNA binding assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Weak affinity supports the proposed quality-control mechanism; it does not prove absolute exclusion under every condition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Quality control reduces entry of the unfinished intermediate into protein synthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Biogenesis of glutaminyl-mt tRNAGln in human mitochondria. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19805282/ · DOI 10.1073/pnas.0907602106","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8317eb9c-b064-51bd-a636-17b0725688ba","evidence_kind":"source_excerpt","locator":"Lines 394-400","start_line":394,"end_line":400,"excerpt":"## glutamate-elongation-quality-control\nQuality control reduces entry of the unfinished intermediate into protein synthesis.\nThe glutamate-mischarged tRNA(Gln) intermediate bound mitochondrial EF-Tu weakly, supporting exclusion from translation before correction.\nModel: Human mitochondrial tRNA binding assays.\nLimitations: Weak affinity supports the proposed quality-control mechanism; it does not prove absolute exclusion under every condition.\nEvidence access: Primary abstract\nBiogenesis of glutaminyl-mt tRNAGln in human mitochondria. · 2009 · https://pubmed.ncbi.nlm.nih.gov/19805282/ · DOI 10.1073/pnas.0907602106","model_system":"Human mitochondrial tRNA binding assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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