{"id":"2c278662-cfa1-5787-8dee-ff11a5f4ce4c","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-tcell-restimulation","predicate":"replacement_requires_renewed_activation","statement":"After tryptophan-sensitive arrest, human T cells required renewed T-cell-receptor signaling as well as tryptophan to resume division.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5e274b98-0922-5ac3-b0f6-6a79fb626f66","mechanism_event_label":"Replacing the nutrient alone did not restore the lost activation commitment.","subject":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"object":{"id":"00a382b3-b785-52fd-8a3f-99253a46d333","slug":"human-activated-t-cell-proliferation","display_name":"Human activated T-cell proliferation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5e274b98-0922-5ac3-b0f6-6a79fb626f66","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-tcell-restimulation-event","event_type":"observed_relationship","label":"Replacing the nutrient alone did not restore the lost activation commitment.","description":"After tryptophan-sensitive arrest, human T cells required renewed T-cell-receptor signaling as well as tryptophan to resume division.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"00a382b3-b785-52fd-8a3f-99253a46d333","slug":"human-activated-t-cell-proliferation","display_name":"Human activated T-cell proliferation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, TCR-dependence results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human T-cell withdrawal, add-back and receptor-restimulation experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A cell-cycle checkpoint experiment, not a general human supplementation rule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Replacing the nutrient alone did not restore the lost activation commitment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of T cell proliferation by macrophage tryptophan catabolism. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10224276/ · DOI 10.1084/jem.189.9.1363","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f4a274f4-8d20-534d-8112-af259aac91b2","evidence_kind":"source_excerpt","locator":"Lines 362-368","start_line":362,"end_line":368,"excerpt":"## tryptophan-tcell-restimulation\nReplacing the nutrient alone did not restore the lost activation commitment.\nAfter tryptophan-sensitive arrest, human T cells required renewed T-cell-receptor signaling as well as tryptophan to resume division.\nModel: Human T-cell withdrawal, add-back and receptor-restimulation experiments.\nLimitations: A cell-cycle checkpoint experiment, not a general human supplementation rule.\nEvidence access: Primary full text, TCR-dependence results\nInhibition of T cell proliferation by macrophage tryptophan catabolism. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10224276/ · DOI 10.1084/jem.189.9.1363","model_system":"Human T-cell withdrawal, add-back and receptor-restimulation experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}