{"id":"2beb94f5-1a4e-51c5-9452-4e78cab2a949","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a15-ergo","predicate":"transports","statement":"Human SLC22A15 expression enabled uptake of ergothioneine in HEK293 substrate assays.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f8cf4a4e-4a1f-5458-93d9-a0a6d6b61b9d","mechanism_event_label":"An additional carrier connects ergothioneine to the transport network.","subject":{"id":"4cc09c49-4205-5f49-bac8-3fb3d23242fc","slug":"slc22a15","display_name":"Human solute carrier SLC22A15","entity_type_key":"protein"},"object":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f8cf4a4e-4a1f-5458-93d9-a0a6d6b61b9d","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-slc22a15-ergo-event","event_type":"observed_relationship","label":"An additional carrier connects ergothioneine to the transport network.","description":"Human SLC22A15 expression enabled uptake of ergothioneine in HEK293 substrate assays.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4cc09c49-4205-5f49-bac8-3fb3d23242fc","slug":"slc22a15","display_name":"Human solute carrier SLC22A15","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Metabolomic screening and uptake assays in transfected human cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Higher Km for ergothioneine, carnitine and carnosine than their established carriers; substrate sharing does not prove competition at dietary exposures.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An additional carrier connects ergothioneine to the transport network.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Deorphaning a solute carrier 22 family member, SLC22A15, through functional genomic studies. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33124720/ · DOI 10.1096/fj.202001497R","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Expression enabled uptake in HEK293 substrate assays.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Expression enabled uptake in HEK293 substrate assays.","entity":null}],"evidence":[{"id":"b2257797-ccb6-531f-9cad-d8f1040839f7","evidence_kind":"source_excerpt","locator":"Lines 48-54","start_line":48,"end_line":54,"excerpt":"## ergothioneine-slc22a15-ergo\nAn additional carrier connects ergothioneine to the transport network.\nHuman SLC22A15 expression enabled uptake of ergothioneine in HEK293 substrate assays.\nModel: Metabolomic screening and uptake assays in transfected human cells.\nLimitations: Higher Km for ergothioneine, carnitine and carnosine than their established carriers; substrate sharing does not prove competition at dietary exposures.\nEvidence access: Primary abstract\nDeorphaning a solute carrier 22 family member, SLC22A15, through functional genomic studies. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33124720/ · DOI 10.1096/fj.202001497R","model_system":"Metabolomic screening and uptake assays in transfected human cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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