{"id":"2bdd85c5-6a85-5381-90ba-405bda30441c","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-gsh-cell-rescue","predicate":"protects","statement":"Glutathione and catalase reversed vanadate sensitivity in the susceptible cell line studied.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"631a1053-5283-57eb-9db6-cf7e43049669","mechanism_event_label":"Available reducing or peroxide-removing capacity changed the toxicity response.","subject":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"object":{"id":"c71afd03-60f4-5fb8-b540-438d558bc100","slug":"vanadate-sensitive-cell-viability","display_name":"Viability in the vanadate-sensitive cell-line experiment","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"631a1053-5283-57eb-9db6-cf7e43049669","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-gsh-cell-rescue-event","event_type":"observed_relationship","label":"Available reducing or peroxide-removing capacity changed the toxicity response.","description":"Glutathione and catalase reversed vanadate sensitivity in the susceptible cell line studied.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c71afd03-60f4-5fb8-b540-438d558bc100","slug":"vanadate-sensitive-cell-viability","display_name":"Viability in the vanadate-sensitive cell-line experiment","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Two cell lines with different susceptibilities; identities not provided in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The proposed intracellular peroxovanadium sequence was not established as a universal mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Available reducing or peroxide-removing capacity changed the toxicity response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mechanisms of vanadate-induced cellular toxicity: role of cellular glutathione and NADPH. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12234491/ · DOI 10.1016/s0003-9861(02)00408-3","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"de1196f9-9b63-5095-87ce-fa6332be5e72","evidence_kind":"source_excerpt","locator":"Lines 142-148","start_line":142,"end_line":148,"excerpt":"## vanadium-gsh-cell-rescue\nAvailable reducing or peroxide-removing capacity changed the toxicity response.\nGlutathione and catalase reversed vanadate sensitivity in the susceptible cell line studied.\nModel: Two cell lines with different susceptibilities; identities not provided in accessed abstract.\nLimitations: The proposed intracellular peroxovanadium sequence was not established as a universal mechanism.\nEvidence access: Primary abstract\nMechanisms of vanadate-induced cellular toxicity: role of cellular glutathione and NADPH. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12234491/ · DOI 10.1016/s0003-9861(02)00408-3","model_system":"Two cell lines with different susceptibilities; identities not provided in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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