{"id":"2bc95a38-3978-59fb-9009-f7a4199e639c","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-pool","predicate":"maintains","statement":"CRISPR deletion of NADK2 in human DLD1 cells reduced mitochondrial NADP(H) by more than 80% while whole-cell NADP(H) was not similarly depleted.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a37a350e-2add-5799-9098-19a290bc5839","mechanism_event_label":"The cofactor pool inside mitochondria can fail even when a whole-cell measurement looks adequate.","subject":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"object":{"id":"b4d55b5e-ddf8-546a-b135-038685e761e1","slug":"mitochondrial-nadp-h-content","display_name":"Mitochondrial NADP(H) content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a37a350e-2add-5799-9098-19a290bc5839","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-pool-event","event_type":"observed_relationship","label":"The cofactor pool inside mitochondria can fail even when a whole-cell measurement looks adequate.","description":"CRISPR deletion of NADK2 in human DLD1 cells reduced mitochondrial NADP(H) by more than 80% while whole-cell NADP(H) was not similarly depleted.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b4d55b5e-ddf8-546a-b135-038685e761e1","slug":"mitochondrial-nadp-h-content","display_name":"Mitochondrial NADP(H) content","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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