{"id":"2b8e9b23-b7ee-502f-98a8-9418b65452cc","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-yeast-ppt2-fe-s","predicate":"deletion_reduces","statement":"Yeast PPT2 deletion, preventing mitochondrial ACP phosphopantetheinylation, reduced aconitase activity; a lipoate-synthesis LIP5 deletion control retained normal activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"5106885a-1e1f-52d5-b2dc-5c6a557cbe1c","mechanism_event_label":"Failure to install the carrier arm affected an iron–sulfur enzyme beyond the lipoate pathway.","subject":{"id":"01339d3c-4e25-5861-9407-4a698e09a86b","slug":"yeast-ppt2","display_name":"Saccharomyces cerevisiae mitochondrial phosphopantetheinyl transferase / Ppt2","entity_type_key":"protein"},"object":{"id":"aa783e22-04fc-5263-9ac2-bb643d45ff08","slug":"cellular-aconitase-activity","display_name":"Total cellular aconitase activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5106885a-1e1f-52d5-b2dc-5c6a557cbe1c","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-met-yeast-ppt2-fe-s-event","event_type":"biochemical_relationship","label":"Failure to install the carrier arm affected an iron–sulfur enzyme beyond the lipoate pathway.","description":"Yeast PPT2 deletion, preventing mitochondrial ACP phosphopantetheinylation, reduced aconitase activity; a lipoate-synthesis LIP5 deletion control retained normal activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"630ff358-f141-5262-891f-f902d1259d65","slug":"yeast-acp1","display_name":"Saccharomyces cerevisiae mitochondrial acyl carrier protein / Acp1","entity_type_key":"protein"},"role":"carrier_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"aab78666-4f68-5db2-b86e-a3c6b18ed62d","slug":"phosphopantetheine","display_name":"4′-Phosphopantetheine","entity_type_key":"small_molecule"},"role":"uninstalled_prosthetic_group","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8058ae47-b091-5004-ba65-1e50ad91f70b","slug":"iron-sulfur-4fe4s","display_name":"[4Fe-4S] iron-sulfur cluster","entity_type_key":"chemical_species"},"role":"aconitase_cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"separate_control_pathway","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"01339d3c-4e25-5861-9407-4a698e09a86b","slug":"yeast-ppt2","display_name":"Saccharomyces cerevisiae mitochondrial phosphopantetheinyl transferase / Ppt2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"aa783e22-04fc-5263-9ac2-bb643d45ff08","slug":"cellular-aconitase-activity","display_name":"Total cellular aconitase activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Full text line 181; Fig. 4C–E","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Yeast PPT2-deletion and LIP5-deletion comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Genetic deletion of PPT2, compared with wild type and LIP5 deletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Aconitase activity is the measured endpoint. The separate sulfite-reductase result is retained in the source, without duplicating it as another claim. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Saccharomyces cerevisiae","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Failure to install the carrier arm affected an iron–sulfur enzyme beyond the lipoate pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. 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The separate sulfite-reductase result is retained in the source, without duplicating it as another claim. This experiment does not establish a dietary pantothenate threshold or benefit from B5 supplementation.\nexposure: Genetic deletion of PPT2, compared with wild type and LIP5 deletion.\ncross_nutrient: true\nevidence_location: Full text line 181; Fig. 4C–E\n[b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. (2016). https://pubmed.ncbi.nlm.nih.gov/27540631/ DOI: 10.7554/elife.17828","model_system":"Yeast PPT2-deletion and LIP5-deletion comparison","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-met-acp2016] The mitochondrial acyl carrier protein (ACP) coordinates mitochondrial fatty acid synthesis with iron sulfur cluster biogenesis. 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