{"id":"2b0f8271-1b6f-5fb6-b9ee-2fd3c46e5355","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-acute","predicate":"increases_phosphorylation","statement":"Acute alanine treatment increased AMPK and downstream ACC phosphorylation in rat H4IIE liver cells over the tested 0.25–10 mM range.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6e7f1f3c-8197-5b45-928e-1318dbca3820","mechanism_event_label":"Alanine metabolism can generate an energy-stress signal in this experimental setting.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"2f112afe-1ecd-54a0-a90d-a8c6ac5cecb6","slug":"rat-ampk-complex","display_name":"Rat AMP-activated protein kinase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"6e7f1f3c-8197-5b45-928e-1318dbca3820","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-acute-event","event_type":"observed_relationship","label":"Alanine metabolism can generate an energy-stress signal in this experimental setting.","description":"Acute alanine treatment increased AMPK and downstream ACC phosphorylation in rat H4IIE liver cells over the tested 0.25–10 mM range.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2f112afe-1ecd-54a0-a90d-a8c6ac5cecb6","slug":"rat-ampk-complex","display_name":"Rat AMP-activated protein kinase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d47321ac-44bb-5a88-afdb-6435b57a91b9","slug":"amp","display_name":"AMP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat H4IIE cells; acute millimolar alanine exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Phosphorylation in culture is not proof of clinical benefit or a universal response to dietary alanine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Alanine metabolism can generate an energy-stress signal in this experimental setting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"l-Alanine activates hepatic AMP-activated protein kinase and modulates systemic glucose metabolism. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30190193/ · DOI 10.1016/j.molmet.2018.08.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f1767a9b-7bee-5423-aeff-1672a6b21417","evidence_kind":"source_excerpt","locator":"Lines 248-254","start_line":248,"end_line":254,"excerpt":"## alanine-ampk-acute\nAlanine metabolism can generate an energy-stress signal in this experimental setting.\nAcute alanine treatment increased AMPK and downstream ACC phosphorylation in rat H4IIE liver cells over the tested 0.25–10 mM range.\nModel: Rat H4IIE cells; 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