{"id":"2b0bd5b8-1d1f-5a84-976c-5834ce3bea70","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:dads-gsh","predicate":"reported_relationship","statement":"Purified DADS released only a small amount of sulfide slowly with GSH; DATS impurity can explain apparent rapid DADS release.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"6c8787b2-5a36-5aba-a78d-b3ad5e3e11e7","mechanism_event_label":"Purified DADS released only a small amount of sulfide slowly with GSH; DATS impurity can explain apparent rapid DADS release.","subject":{"id":"a2c4385d-accb-581b-9268-c851cb60cbab","slug":"diallyl-disulfide","display_name":"Diallyl disulfide / DADS","entity_type_key":"small_molecule"},"object":{"id":"03afb94f-0a27-5627-9882-1dcb1f44c754","slug":"dads-gsh-sulfide-production","display_name":"Sulfide generation in the DADS plus GSH chemical assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6c8787b2-5a36-5aba-a78d-b3ad5e3e11e7","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:dads-gsh-event","event_type":"biochemical_relationship","label":"Purified DADS released only a small amount of sulfide slowly with GSH; DATS impurity can explain apparent rapid DADS release.","description":"**DATS and DADS differ.** A later chemical study found rapid GSH-dependent sulfide release from DATS, involving an allyl-perthiol intermediate, but very little and slow release from DADS. DATS contamination could account for fast release attributed to DADS. Sulfur-chain length, purity, and reaction conditions belong in the record. This qualifies older attribution without deleting either observation; it does not establish that allicin or aged garlic extract has the same behavior. [Liang 2015](https://pubmed.ncbi.nlm.nih.gov/26301500/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a2c4385d-accb-581b-9268-c851cb60cbab","slug":"diallyl-disulfide","display_name":"Diallyl disulfide / DADS","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"03afb94f-0a27-5627-9882-1dcb1f44c754","slug":"dads-gsh-sulfide-production","display_name":"Sulfide generation in the DADS plus GSH chemical assay","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"reacting thiol","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"70cc3ced-8adc-5e76-9750-e1415683455b","slug":"hydrogen-sulfide","display_name":"Hydrogen sulfide / H2S","entity_type_key":"small_molecule"},"role":"measured product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"267dc359-d357-5af4-9b4a-d4cf3f7360fc","slug":"diallyl-trisulfide","display_name":"Diallyl trisulfide / DATS","entity_type_key":"small_molecule"},"role":"identified attribution confounder","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified-compound comparison; source-specific purity interpretation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Slow/minute is not zero. Historical observations remain preserved with purity qualification, not erased as fictional experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Purified DADS released only a small amount of sulfide slowly with GSH; DATS impurity can explain apparent rapid DADS release.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Diallyl Trisulfide Is a Fast H2S Donor, but Diallyl Disulfide Is a Slow One: The Reaction Pathways and Intermediates of Glutathione with Polysulfides. | 2015 | DOI 10.1021/acs.orglett.5b01962 | PMID 26301500 | https://pubmed.ncbi.nlm.nih.gov/26301500/ | https://doi.org/10.1021/acs.orglett.5b01962","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 83-83; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"13c46f01-6150-5f34-9599-a36e3b1cee3d","evidence_kind":"source_excerpt","locator":"Lines 83-83","start_line":83,"end_line":83,"excerpt":"**DATS and DADS differ.** A later chemical study found rapid GSH-dependent sulfide release from DATS, involving an allyl-perthiol intermediate, but very little and slow release from DADS. DATS contamination could account for fast release attributed to DADS. Sulfur-chain length, purity, and reaction conditions belong in the record. This qualifies older attribution without deleting either observation; it does not establish that allicin or aged garlic extract has the same behavior. [Liang 2015](https://pubmed.ncbi.nlm.nih.gov/26301500/)","model_system":"Purified-compound comparison; source-specific purity interpretation.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}