{"id":"2a929903-95f7-5495-a067-aa24a287f28c","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-ra-mouse-stra8","predicate":"induces_expression","statement":"All-trans RA induced Stra8 RNA in cultured fetal mouse testes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"878e4674-0db8-5fa5-8b20-b8ef18b70447","mechanism_event_label":"RA can turn on Stra8 in fetal testicular germ cells.","subject":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"6e6d852f-648d-5e5b-82a8-5a02fa77b14b","slug":"mouse-stra8-expression","display_name":"Mouse Stra8 expression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"878e4674-0db8-5fa5-8b20-b8ef18b70447","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-ra-mouse-stra8-event","event_type":"biochemical_relationship","label":"RA can turn on Stra8 in fetal testicular germ cells.","description":"All-trans RA induced Stra8 RNA in cultured fetal mouse testes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cdfcd658-889e-5002-8167-15af0f8130ae","slug":"mouse-stra8","display_name":"Mouse STRA8","entity_type_key":"protein"},"role":"encoded_regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6e6d852f-648d-5e5b-82a8-5a02fa77b14b","slug":"mouse-stra8-expression","display_name":"Mouse Stra8 expression","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Embryonic gonad culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"E12.5 testes; 0.7 micromolar RA for 2 days.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"RNA induction alone did not establish completed meiosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"RA can turn on Stra8 in fetal testicular germ cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[koubova2006] Retinoic acid regulates sex-specific timing of meiotic initiation in mice. (2006). https://pubmed.ncbi.nlm.nih.gov/16461896/ DOI: 10.1073/pnas.0510813103","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"fetal testis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7555ba43-e74e-51e8-9ae2-d9b5a52c9bfb","evidence_kind":"source_excerpt","locator":"Lines 161-172","start_line":161,"end_line":172,"excerpt":"### va-repro-ra-mouse-stra8\nAll-trans RA induced Stra8 RNA in cultured fetal mouse testes.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RA can turn on Stra8 in fetal testicular germ cells.\norganism: Mus musculus\ntissue_or_cell_type: fetal testis\nexperimental_model: Embryonic gonad culture\nlimitations: RNA induction alone did not establish completed meiosis.\nexposure: E12.5 testes; 0.7 micromolar RA for 2 days.\ncross_nutrient: false\n[koubova2006] Retinoic acid regulates sex-specific timing of meiotic initiation in mice. (2006). https://pubmed.ncbi.nlm.nih.gov/16461896/ DOI: 10.1073/pnas.0510813103","model_system":"Embryonic gonad culture","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [koubova2006] Retinoic acid regulates sex-specific timing of meiotic initiation in mice. (2006). https://pubmed.ncbi.nlm.nih.gov/16461896/ DOI: 10.1073/pnas.0510813103","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"83131a07-353f-5301-9ce6-50754c194952","stable_key":"import-3c852f36-7951-5fd9-9013-d771a8d16d8a","title":"Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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