{"id":"2a915a65-5634-5cce-be31-4480bcde5e01","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-bmp-expression","predicate":"increases_expression","statement":"Ten micromolar orthosilicic acid increased BMP2 expression in human osteoblast-like cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"13c0c1a1-00b4-59a6-ad6c-1519f25aa6f3","mechanism_event_label":"A bone-development signal increased after exposure.","subject":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"object":{"id":"a2b4dc1b-5982-501a-836a-00692b9fddea","slug":"human-bmp2","display_name":"Human BMP2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"13c0c1a1-00b4-59a6-ad6c-1519f25aa6f3","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-bmp-expression-event","event_type":"observed_relationship","label":"A bone-development signal increased after exposure.","description":"Ten micromolar orthosilicic acid increased BMP2 expression in human osteoblast-like cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a2b4dc1b-5982-501a-836a-00692b9fddea","slug":"human-bmp2","display_name":"Human BMP2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human MG-63/U2-OS cell program.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression change does not identify a direct silicon receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A bone-development signal increased after exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Biological Silicon Stimulates Collagen Type 1 and Osteocalcin Synthesis in Human Osteoblast-Like Cells Through the BMP-2/Smad/RUNX2 Signaling Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27025722/ · DOI 10.1007/s12011-016-0686-3","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7800c444-8b16-5ffa-b7c6-99fa4bc73e89","evidence_kind":"source_excerpt","locator":"Lines 144-150","start_line":144,"end_line":150,"excerpt":"## silica-bmp-expression\nA bone-development signal increased after exposure.\nTen micromolar orthosilicic acid increased BMP2 expression in human osteoblast-like cells.\nModel: Human MG-63/U2-OS cell program.\nLimitations: Expression change does not identify a direct silicon receptor.\nEvidence access: Primary abstract\nBiological Silicon Stimulates Collagen Type 1 and Osteocalcin Synthesis in Human Osteoblast-Like Cells Through the BMP-2/Smad/RUNX2 Signaling Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27025722/ · DOI 10.1007/s12011-016-0686-3","model_system":"Human MG-63/U2-OS cell program.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"e05d81962a1ca6cbfc68fce4fd3dbcdea67c8e61332c19f4fec5d0c3ab8bf1b9","revision_id":"6433ed49-a331-571b-a611-21e56c3ce4bc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}