{"id":"2a8c347b-50a2-57c3-a49f-ef623a36fcb3","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-syt-binding","predicate":"forms_phospholipid_complex_with","statement":"In the synaptotagmin-1 domain assays, strontium supported C2B/phospholipid complexes but not C2A/phospholipid complexes, whereas calcium supported both.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8362c006-8743-59c4-9e2b-25b0fe263e19","mechanism_event_label":"Different domains of a calcium sensor discriminate between the ions.","subject":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"object":{"id":"4652a3ed-3684-5fe8-8e4d-99a62f4f1822","slug":"synaptotagmin1-c2b-sr-assay","display_name":"Synaptotagmin-1 C2B domain in the strontium-binding assay","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"8362c006-8743-59c4-9e2b-25b0fe263e19","stable_key":"1ff3a29f-ef80-5bff-9300-30b98c60b90d:strontium-syt-binding-event","event_type":"observed_relationship","label":"Different domains of a calcium sensor discriminate between the ions.","description":"In the synaptotagmin-1 domain assays, strontium supported C2B/phospholipid complexes but not C2A/phospholipid complexes, whereas calcium supported both.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"97607549-3a37-544e-b6e6-266887f7c35f","slug":"strontium-ion","display_name":"Strontium ion / Sr2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4652a3ed-3684-5fe8-8e4d-99a62f4f1822","slug":"synaptotagmin1-c2b-sr-assay","display_name":"Synaptotagmin-1 C2B domain in the strontium-binding assay","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2ca2864b-1ef0-54e4-8397-1dbe7207a0d2","slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical domain/phospholipid binding accompanying mouse neuronal experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Accessed abstract does not identify the species of recombinant domain constructs; that identity remains unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Strontium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"strontium","display_name":"Strontium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Different domains of a calcium sensor discriminate between the ions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12526776/ · DOI 10.1016/s0896-6273(02)01145-5","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1aef259c-c9fb-5cba-a146-5839b8c946c7","evidence_kind":"source_excerpt","locator":"Lines 358-364","start_line":358,"end_line":364,"excerpt":"## strontium-syt-binding\nDifferent domains of a calcium sensor discriminate between the ions.\nIn the synaptotagmin-1 domain assays, strontium supported C2B/phospholipid complexes but not C2A/phospholipid complexes, whereas calcium supported both.\nModel: Biochemical domain/phospholipid binding accompanying mouse neuronal experiments.\nLimitations: Accessed abstract does not identify the species of recombinant domain constructs; that identity remains unresolved.\nEvidence access: Primary abstract\nSr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12526776/ · DOI 10.1016/s0896-6273(02)01145-5","model_system":"Biochemical domain/phospholipid binding accompanying mouse neuronal experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"43ef0fe7-eb6c-5346-8381-f7694733b4dc","stable_key":"import-1ff3a29f-ef80-5bff-9300-30b98c60b90d","title":"Strontium: calcium interactions, cellular mechanisms and mineralization (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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