{"id":"2a3fc101-013f-5827-a2c7-44d1f5d00c43","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-g6pd-fmn-oxidase-response","predicate":"improved_low_fmn_dependent_oxidase_activity","statement":"Low FMN-dependent PNP oxidase activity in many G6PD-deficient participants responded to oral riboflavin despite the contrasting FAD-saturated GSR pattern.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0306f390-df93-5bd6-8cfc-404ca038cf09","mechanism_event_label":"FMN and FAD should not be treated as one interchangeable vitamin pool.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"dda07945-be77-56d7-914b-61a1bffeca9e","slug":"erythrocyte-pnpo-activity","display_name":"Erythrocyte pyridoxamine/pyridoxine phosphate oxidase activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0306f390-df93-5bd6-8cfc-404ca038cf09","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-g6pd-fmn-oxidase-response-event","event_type":"observed_intervention","label":"FMN and FAD should not be treated as one interchangeable vitamin pool.","description":"Low FMN-dependent PNP oxidase activity in many G6PD-deficient participants responded to oral riboflavin despite the contrasting FAD-saturated GSR pattern.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a01d08b9-a749-5bc3-934b-af0b35024b54","slug":"pnpo","display_name":"Pyridoxine 5-prime-phosphate oxidase / PNPO","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d3eacf85-1a35-546d-bd30-161474dd9691","slug":"fmn","display_name":"Flavin mononucleotide","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f65184d1-6b79-5e38-8137-a06a40e94e36","slug":"g6pd","display_name":"Glucose-6-phosphate dehydrogenase / G6PD","entity_type_key":"protein"},"role":"genetic_context","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"dda07945-be77-56d7-914b-61a1bffeca9e","slug":"erythrocyte-pnpo-activity","display_name":"Erythrocyte pyridoxamine/pyridoxine phosphate oxidase activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"B2 cofactor supply affects a B6-activation assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human erythrocytes from G6PD deficiency, heterozygous beta-thalassemia and controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"In-vitro FAD stimulation of glutathione reductase and PNP oxidase activity, with oral-riboflavin response observations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No isolated whole-body B6 flux or clinical seizure/neuropathy outcome was established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"FMN and FAD should not be treated as one interchangeable vitamin pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b2-anderson1987] Glutathione reductase activity and its relationship to pyridoxine phosphate activity in G6PD deficiency (1987). https://pubmed.ncbi.nlm.nih.gov/3582603/ DOI: 10.1111/j.1600-0609.1987.tb01417.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human clinical setting","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"9d7d763f-914e-502c-a4ed-be7ef872cf5b","evidence_kind":"source_excerpt","locator":"Lines 1679-1690","start_line":1679,"end_line":1690,"excerpt":"### b2-g6pd-fmn-oxidase-response\nLow FMN-dependent PNP oxidase activity in many G6PD-deficient participants responded to oral riboflavin despite the contrasting FAD-saturated GSR pattern.\nCondition category: biomarker_context\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: FMN and FAD should not be treated as one interchangeable vitamin pool.\norganism: Homo sapiens\ntissue_or_cell_type: Human clinical setting\nexperimental_model: Human erythrocytes from G6PD deficiency, heterozygous beta-thalassemia and controls.\nlimitations: No isolated whole-body B6 flux or clinical seizure/neuropathy outcome was established.\nexposure: In-vitro FAD stimulation of glutathione reductase and PNP oxidase activity, with oral-riboflavin response observations.\ncross_nutrient: B2 cofactor supply affects a B6-activation assay.\n[b2-anderson1987] Glutathione reductase activity and its relationship to pyridoxine phosphate activity in G6PD deficiency (1987). https://pubmed.ncbi.nlm.nih.gov/3582603/ DOI: 10.1111/j.1600-0609.1987.tb01417.x","model_system":"Human erythrocytes from G6PD deficiency, heterozygous beta-thalassemia and controls.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b2-anderson1987] Glutathione reductase activity and its relationship to pyridoxine phosphate activity in G6PD deficiency (1987). https://pubmed.ncbi.nlm.nih.gov/3582603/ DOI: 10.1111/j.1600-0609.1987.tb01417.x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}