{"id":"29feb8f1-20d3-5169-8a88-f2bef2341f7f","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:trpv6-loss-reduces-absorption","predicate":"loss_of_function_reduces","statement":"Trpv6 deletion reduced intestinal calcium absorption by about 60% in the Bianco mouse model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c838855b-5dd0-54ed-82d7-c57129ba4893","mechanism_event_label":"TRPV6 materially supports calcium uptake in this model.","subject":{"id":"2f5ca870-de94-5d5e-a9f6-d8da99e26360","slug":"trpv6","display_name":"TRPV6","entity_type_key":"protein"},"object":{"id":"53d44ad2-3648-5c31-8328-bcc1344961df","slug":"intestinal-calcium-absorption","display_name":"Intestinal calcium absorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c838855b-5dd0-54ed-82d7-c57129ba4893","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:trpv6-loss-reduces-absorption-event","event_type":"biochemical_relationship","label":"TRPV6 materially supports calcium uptake in this model.","description":"Trpv6 deletion reduced intestinal calcium absorption by about 60% in the Bianco mouse model.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2f5ca870-de94-5d5e-a9f6-d8da99e26360","slug":"trpv6","display_name":"TRPV6","entity_type_key":"protein"},"role":"impaired_channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_ion","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53d44ad2-3648-5c31-8328-bcc1344961df","slug":"intestinal-calcium-absorption","display_name":"Intestinal calcium absorption","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Whole-body Trpv6 knockout mice under regular and low-calcium diets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Whole-body deletion; diet and assay matter; residual absorption remained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"TRPV6 materially supports calcium uptake in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[bianco2007] Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene (2007). https://pubmed.ncbi.nlm.nih.gov/17129178/ DOI: 10.1359/jbmr.061110","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f50482bd-7414-5abf-b5be-85b1516b86ef","evidence_kind":"source_excerpt","locator":"Lines 158-167","start_line":158,"end_line":167,"excerpt":"### trpv6-loss-reduces-absorption\nTrpv6 deletion reduced intestinal calcium absorption by about 60% in the Bianco mouse model.\nCondition category: machinery_impairment\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: TRPV6 materially supports calcium uptake in this model.\norganism: Mus musculus\ntissue_or_cell_type: Intestine\nexperimental_model: Whole-body Trpv6 knockout mice under regular and low-calcium diets\nlimitations: Whole-body deletion; diet and assay matter; residual absorption remained.\n[bianco2007] Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene (2007). https://pubmed.ncbi.nlm.nih.gov/17129178/ DOI: 10.1359/jbmr.061110","model_system":"Whole-body Trpv6 knockout mice under regular and low-calcium diets","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [bianco2007] Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene (2007). https://pubmed.ncbi.nlm.nih.gov/17129178/ DOI: 10.1359/jbmr.061110","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[{"id":"fb59b6c7-4bfc-591a-8693-b00553017af8","title":"TRPV6 supports absorption but is not the only route","kind":"context_difference","status":"qualified","why":"Trpv6 deletion reduced uptake in one model, while significant active transport persisted without TRPV6 and calbindin in another.","resolution":"Curation interpretation: Keep genotype, diet and assay conditions attached. Contribution does not establish absolute necessity.","created_at":"2026-09-17 08:08:54","record_type":"qualification","display_label":"Source qualification","record_url":"/corrections/fb59b6c7-4bfc-591a-8693-b00553017af8","sides":[{"conflict_id":"fb59b6c7-4bfc-591a-8693-b00553017af8","ordinal":0,"label":"TRPV6 materially supports calcium uptake in this model.","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","start_line":158,"end_line":167,"quote":"### trpv6-loss-reduces-absorption\nTrpv6 deletion reduced intestinal calcium absorption by about 60% in the Bianco mouse model.\nCondition category: machinery_impairment\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: TRPV6 materially supports calcium uptake in this model.\norganism: Mus musculus\ntissue_or_cell_type: Intestine\nexperimental_model: Whole-body Trpv6 knockout mice under regular and low-calcium diets\nlimitations: Whole-body deletion; diet and assay matter; residual absorption remained.\n[bianco2007] Marked disturbance of calcium homeostasis in mice with targeted disruption of the Trpv6 calcium channel gene (2007). https://pubmed.ncbi.nlm.nih.gov/17129178/ DOI: 10.1359/jbmr.061110","source_key":"import-434e1e27-eb90-583a-956b-62472726ffba","source_title":"Calcium: mechanism-first literature curation (2026-09-17)","claim_ids":["29feb8f1-20d3-5169-8a88-f2bef2341f7f"]},{"conflict_id":"fb59b6c7-4bfc-591a-8693-b00553017af8","ordinal":1,"label":"Other routes can support active calcium absorption.","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","start_line":169,"end_line":179,"quote":"### trpv6-calbindin-independent-active-transport\nSignificant active duodenal calcium transport persists in Trpv6/calbindin-D9k double-null mice under the tested stimulation conditions.\nCondition category: machinery_impairment\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Other routes can support active calcium absorption.\norganism: Mus musculus\ntissue_or_cell_type: Duodenum\nexperimental_model: Trpv6 and calbindin-D9k single/double knockout mice; duodenal transport assays\nlimitations: Residual transport does not establish dispensability under every diet or life stage.\nreported_effect: retained\n[benn2008] Active intestinal calcium transport in the absence of transient receptor potential vanilloid type 6 and calbindin-D9k (2008). https://pmc.ncbi.nlm.nih.gov/articles/PMC2408805/ DOI: 10.1210/en.2007-1655","source_key":"import-434e1e27-eb90-583a-956b-62472726ffba","source_title":"Calcium: mechanism-first literature curation (2026-09-17)","claim_ids":["00679d3e-56aa-5de4-9d6c-9f98cad99df2"]}]}],"research":null}