{"id":"29fd02ac-d542-5eca-8249-1be46a1c209c","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-stim1-knockdown-influx","predicate":"supports","statement":"STIM1 knockdown suppresses store-depletion-triggered calcium influx in HeLa cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5a1555de-2502-52b5-8356-d58cace6c38c","mechanism_event_label":"Reducing STIM1 weakens calcium entry after stores empty.","subject":{"id":"f143762c-e7f0-54de-8ae3-cb2dcda9040e","slug":"stim1","display_name":"STIM1","entity_type_key":"protein"},"object":{"id":"a3169279-41ce-5c17-919f-dc7b48f6f6d6","slug":"store-operated-calcium-entry","display_name":"Store-operated calcium entry","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5a1555de-2502-52b5-8356-d58cace6c38c","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-stim1-knockdown-influx-event","event_type":"loss_of_function","label":"Reducing STIM1 weakens calcium entry after stores empty.","description":"STIM1 knockdown suppresses store-depletion-triggered calcium influx in HeLa cells.","status":"provisional","compartment":{"slug":"er-membrane","display_name":"ER membrane"},"participants":[{"entity":{"id":"f143762c-e7f0-54de-8ae3-cb2dcda9040e","slug":"stim1","display_name":"STIM1","entity_type_key":"protein"},"role":"impaired_sensor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a3169279-41ce-5c17-919f-dc7b48f6f6d6","slug":"store-operated-calcium-entry","display_name":"Store-operated calcium entry","entity_type_key":"cellular_process"},"role":"affected_process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7df03d06-a2b7-524a-aa99-c9cf6a3bf4c2","slug":"er-membrane","display_name":"ER membrane","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Study location; 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topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Reducing STIM1 weakens calcium entry after stores empty.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-liou2005] STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx (2005). https://pubmed.ncbi.nlm.nih.gov/16005298/ DOI: 10.1016/j.cub.2005.05.055","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"loss_of_function","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HeLa cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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Study references: [ca-liou2005] STIM is a Ca2+ sensor essential for Ca2+-store-depletion-triggered Ca2+ influx (2005). https://pubmed.ncbi.nlm.nih.gov/16005298/ DOI: 10.1016/j.cub.2005.05.055","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}