{"id":"29dc84fc-c8cc-511b-a1e9-372c13d10177","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nos2-inhibition","predicate":"competitively_inhibits","statement":"Agmatine competitively inhibited macrophage-derived NOS II with approximate Ki 220 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"528e9fc5-42ab-52ad-b8b8-8463c1be0a12","mechanism_event_label":"NOS isoforms showed different assay sensitivities.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"5b00351c-d380-523c-afde-af390dd0592e","slug":"nos2-1996-agmatine-assay","display_name":"Macrophage-derived NOS II in the 1996 agmatine assay, species unverified","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"528e9fc5-42ab-52ad-b8b8-8463c1be0a12","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nos2-inhibition-event","event_type":"observed_relationship","label":"NOS isoforms showed different assay sensitivities.","description":"Agmatine competitively inhibited macrophage-derived NOS II with approximate Ki 220 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5b00351c-d380-523c-afde-af390dd0592e","slug":"nos2-1996-agmatine-assay","display_name":"Macrophage-derived NOS II in the 1996 agmatine assay, species unverified","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f53c7bfb-3b91-56fe-bebd-28623cfe13a0","slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Macrophage NOS preparation; enzymatic activity assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is not a universal inflammatory-cell response or a human concentration target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"NOS isoforms showed different assay sensitivities.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of mammalian nitric oxide synthases by agmatine, an endogenous polyamine formed by decarboxylation of arginine. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8645212/ · DOI 10.1042/bj3160247","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6928c7c2-5ceb-5c8f-8876-3fe5aab76a60","evidence_kind":"source_excerpt","locator":"Lines 228-234","start_line":228,"end_line":234,"excerpt":"## agmatine-sulfate-nos2-inhibition\nNOS isoforms showed different assay sensitivities.\nAgmatine competitively inhibited macrophage-derived NOS II with approximate Ki 220 micromolar.\nModel: Macrophage NOS preparation; enzymatic activity assay.\nLimitations: This is not a universal inflammatory-cell response or a human concentration target.\nEvidence access: Primary abstract\nInhibition of mammalian nitric oxide synthases by agmatine, an endogenous polyamine formed by decarboxylation of arginine. · 1996 · https://pubmed.ncbi.nlm.nih.gov/8645212/ · DOI 10.1042/bj3160247","model_system":"Macrophage NOS preparation; enzymatic activity assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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