{"id":"29c25a98-36a4-5425-ba43-0de7dc128a27","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-norathyriol-ugt1a7","predicate":"competitively_inhibits","statement":"Norathyriol competitively inhibited UGT1A7; IC50 4.4 and Ki 2.0 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fa440fd4-b93c-59d1-85bf-ef792dfedc8a","mechanism_event_label":"The aglycone has its own conjugation-enzyme interaction profile.","subject":{"id":"d0f7f42a-ac45-5144-9ea5-a7586896cc6e","slug":"norathyriol","display_name":"Norathyriol","entity_type_key":"small_molecule"},"object":{"id":"7a22f773-465d-583b-a733-12fcab02636a","slug":"ugt1a7","display_name":"Human UDP-glucuronosyltransferase 1A7 / UGT1A7","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fa440fd4-b93c-59d1-85bf-ef792dfedc8a","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-norathyriol-ugt1a7-event","event_type":"biochemical_relationship","label":"The aglycone has its own conjugation-enzyme interaction profile.","description":"Norathyriol competitively inhibited UGT1A7; IC50 4.4 and Ki 2.0 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d0f7f42a-ac45-5144-9ea5-a7586896cc6e","slug":"norathyriol","display_name":"Norathyriol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7a22f773-465d-583b-a733-12fcab02636a","slug":"ugt1a7","display_name":"Human UDP-glucuronosyltransferase 1A7 / UGT1A7","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/28621744.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\", \"start_char\": 0, \"end_char\": 1562, \"text_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant UGT inhibition kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Micromolar norathyriol; initial comparison at 100 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Probe-dependent biochemical inhibition; no established human interaction threshold. Docking is not additional proof of binding in vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Recombinant enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The aglycone has its own conjugation-enzyme interaction profile.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p28621744] In Vitro Comparative Study of the Inhibitory Effects of Mangiferin and Its Aglycone Norathyriol towards UDP-Glucuronosyl Transferase (UGT) Isoforms. (2017). https://pubmed.ncbi.nlm.nih.gov/28621744/ DOI: 10.3390/molecules22061008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"4-methylumbelliferone glucuronidation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"75cecfef-d7a9-5463-bbb2-71c974771d49","evidence_kind":"source_excerpt","locator":"Lines 887-898","start_line":887,"end_line":898,"excerpt":"### mangiferin-norathyriol-ugt1a7\nNorathyriol competitively inhibited UGT1A7; IC50 4.4 and Ki 2.0 micromolar.\nCondition category: normal\nnutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The aglycone has its own conjugation-enzyme interaction profile.\norganism: Recombinant enzyme assay\ntissue_or_cell_type: 4-methylumbelliferone glucuronidation\nexperimental_model: Recombinant UGT inhibition kinetics\nlimitations: Probe-dependent biochemical inhibition; no established human interaction threshold. Docking is not additional proof of binding in vivo.\nexposure: Micromolar norathyriol; initial comparison at 100 micromolar\nevidence_span: {\"source_cache\": \"artifacts/mangiferin-research/28621744.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\", \"start_char\": 0, \"end_char\": 1562, \"text_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\"}\n[mangiferin-p28621744] In Vitro Comparative Study of the Inhibitory Effects of Mangiferin and Its Aglycone Norathyriol towards UDP-Glucuronosyl Transferase (UGT) Isoforms. (2017). https://pubmed.ncbi.nlm.nih.gov/28621744/ DOI: 10.3390/molecules22061008","model_system":"Recombinant UGT inhibition kinetics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mangiferin-p28621744] In Vitro Comparative Study of the Inhibitory Effects of Mangiferin and Its Aglycone Norathyriol towards UDP-Glucuronosyl Transferase (UGT) Isoforms. (2017). https://pubmed.ncbi.nlm.nih.gov/28621744/ DOI: 10.3390/molecules22061008","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"267f942a-695a-5b62-a858-f71c06321ff5","stable_key":"import-475ea55a-65e9-51c3-a539-738a6a8f683f","title":"Mangiferin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"295cb3a13742b3caeed19347ffcbc7863f5372d983da1625a942428919b4dc98","revision_id":"d9c2ee89-ff22-5e86-9d7f-7cb7e17f9ed2","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}