{"id":"29b68e66-fb6a-58d9-8d96-b27b9dcb8f95","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-leydig-star","predicate":"increases_tested","statement":"Adding 0.1 nM D-aspartate to hCG stimulation increased StAR protein in mouse MA-10 cells compared with hCG alone.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e6b10024-a8f2-543c-96d4-6e2e7ef2847b","mechanism_event_label":"The combination changed a protein involved in steroid production.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"883b062b-cd5e-5bf3-a012-15374c1022f7","slug":"mouse-star","display_name":"Mouse steroidogenic acute regulatory protein / Star","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e6b10024-a8f2-543c-96d4-6e2e7ef2847b","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-leydig-star-event","event_type":"observed_relationship","label":"The combination changed a protein involved in steroid production.","description":"Adding 0.1 nM D-aspartate to hCG stimulation increased StAR protein in mouse MA-10 cells compared with hCG alone.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"883b062b-cd5e-5bf3-a012-15374c1022f7","slug":"mouse-star","display_name":"Mouse steroidogenic acute regulatory protein / Star","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6ee453fb-ad7f-54ca-ab03-6397b781901e","slug":"human-chorionic-gonadotropin","display_name":"Human chorionic gonadotropin","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse MA-10 immunoblot assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Protein abundance does not directly measure cholesterol transfer flux or prove complete mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The combination changed a protein involved in steroid production.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"D-Aspartic acid stimulates steroidogenesis through the delay of LH receptor internalization in a mammalian Leydig cell line. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26122485/ · DOI 10.1007/s40618-015-0333-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4582c4f0-5ce1-5825-955e-49aa1d335b67","evidence_kind":"source_excerpt","locator":"Lines 392-398","start_line":392,"end_line":398,"excerpt":"## d-aspartate-leydig-star\nThe combination changed a protein involved in steroid production.\nAdding 0.1 nM D-aspartate to hCG stimulation increased StAR protein in mouse MA-10 cells compared with hCG alone.\nModel: Mouse MA-10 immunoblot assay.\nLimitations: Protein abundance does not directly measure cholesterol transfer flux or prove complete mediation.\nEvidence access: Primary abstract\nD-Aspartic acid stimulates steroidogenesis through the delay of LH receptor internalization in a mammalian Leydig cell line. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26122485/ · DOI 10.1007/s40618-015-0333-4","model_system":"Mouse MA-10 immunoblot assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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