{"id":"2986c098-c894-50bd-9e4b-64c889b3c5ec","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:renal-high-glucose-thiamine-transport","predicate":"reported_comparison","statement":"High glucose reduced apical-to-basolateral thiamine transport across proximal-tubule monolayers by 37%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c42cab63-a20e-5c03-8936-362f3b9a9ffb","mechanism_event_label":"High glucose reduced apical-to-basolateral thiamine transport across proximal-tubule monolayers by 37%.","subject":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"object":{"id":"a4339716-c8cb-5a7a-a34b-cdfda528b99f","slug":"human-renal-thiamine-reabsorption","display_name":"Human proximal-tubule thiamine reabsorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c42cab63-a20e-5c03-8936-362f3b9a9ffb","stable_key":"3ad23a58-d6a8-5554-81d6-9c83abd33087:renal-high-glucose-thiamine-transport","event_type":"reported_comparison","label":"High glucose reduced apical-to-basolateral thiamine transport across proximal-tubule monolayers by 37%.","description":"","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"experimental_actor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a4339716-c8cb-5a7a-a34b-cdfda528b99f","slug":"human-renal-thiamine-reabsorption","display_name":"Human proximal-tubule thiamine reabsorption","entity_type_key":"cellular_process"},"role":"measured_endpoint","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"36b7c3eb-068b-500f-af1c-078829df1ecc","slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"c9e677e1-db84-51e4-8a27-911614687d5f","slug":"slc19a2","display_name":"Human thiamine transporter 1 / SLC19A2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4eba176e-17c8-5515-920a-df5d48a40486","slug":"slc19a3","display_name":"Human thiamine transporter 2 / SLC19A3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"curation_topic","value_text":"thiamine","comparator":null,"unit":null,"notes":"","entity":{"slug":"thiamine","display_name":"Thiamine (vitamin B1)","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"26 mM glucose","comparator":"5 mM glucose","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"26 mM glucose\", \"comparator\": \"5 mM glucose\", \"endpoint\": \"Directional thiamine transport\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": [{\"entity_slug\": \"glucose\", \"state\": \"26 mM glucose\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Human primary proximal-tubule epithelial cells; 5-day culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport assay; transporter-expression changes accompany the effect without proving exclusive mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Larkin et al. 2012; DOI:10.1371/journal.pone.0053175; PMCID:PMC3532206; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0053175","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e7aeaac0-412d-51fa-8ca1-e9db6212adec","evidence_kind":"source_excerpt","locator":"Lines 24-24","start_line":24,"end_line":24,"excerpt":"High glucose reduced apical-to-basolateral thiamine transport across proximal-tubule monolayers by 37%. Model: Human primary proximal-tubule epithelial cells; 5-day culture. Limits: Transport assay; transporter-expression changes accompany the effect without proving exclusive mediation. Primary reference: Larkin et al. 2012; DOI:10.1371/journal.pone.0053175; PMCID:PMC3532206; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0053175","model_system":"Human primary proximal-tubule epithelial cells; 5-day culture","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase of the cited primary result; see stated access scope.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"8600b90c-477e-569e-9666-09321af7bc9e","stable_key":"import-3ad23a58-d6a8-5554-81d6-9c83abd33087","title":"Diabetes cascade: targeted primary-source supplement","document_type":"imported_text","citation_label":"See claim-local references; curated paraphrases reviewed 2026-09-20.","file_path":"","sha256":"0a841d67ea40a5efea21157e1a5703488b8ef63e587eea50dacac19c67894cd4","revision_id":"0faa1a78-e820-559e-a23b-5e3939f9079e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}