{"id":"29766c82-9f8f-5030-bed2-bc4876f747f5","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-hars-charging","predicate":"forms","statement":"HARS1 ligates histidine to its cognate tRNA for protein synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5d0968e9-d709-538d-8ad5-9d40f5a56309","mechanism_event_label":"A charging enzyme connects free histidine to the protein-building machinery.","subject":{"id":"639ed1bf-e69f-5e4f-b5b1-279638b21bec","slug":"hars1","display_name":"Human cytosolic histidyl-tRNA synthetase / HARS1","entity_type_key":"protein"},"object":{"id":"ef605409-06d8-5bc4-a3df-adb18f780f74","slug":"human-cytosolic-histidyl-trna","display_name":"Human cytosolic histidyl-tRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"5d0968e9-d709-538d-8ad5-9d40f5a56309","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-hars-charging-event","event_type":"observed_relationship","label":"A charging enzyme connects free histidine to the protein-building machinery.","description":"HARS1 ligates histidine to its cognate tRNA for protein synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"639ed1bf-e69f-5e4f-b5b1-279638b21bec","slug":"hars1","display_name":"Human cytosolic histidyl-tRNA synthetase / HARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ef605409-06d8-5bc4-a3df-adb18f780f74","slug":"human-cytosolic-histidyl-trna","display_name":"Human cytosolic histidyl-tRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Established enzymatic role in a primary HARS variant study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Background chemistry rather than a new dietary intervention.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Histidine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A charging enzyme connects free histidine to the protein-building machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A loss-of-function variant in the human histidyl-tRNA synthetase (HARS) gene is neurotoxic in vivo. · 2013 · https://pubmed.ncbi.nlm.nih.gov/22930593/ · DOI 10.1002/humu.22210","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b3ad490e-8832-5b33-9ade-70940a7c9151","evidence_kind":"source_excerpt","locator":"Lines 458-464","start_line":458,"end_line":464,"excerpt":"## histidine-hars-charging\nA charging enzyme connects free histidine to the protein-building machinery.\nHARS1 ligates histidine to its cognate tRNA for protein synthesis.\nModel: Established enzymatic role in a primary HARS variant study.\nLimitations: Background chemistry rather than a new dietary intervention.\nEvidence access: Primary full text\nA loss-of-function variant in the human histidyl-tRNA synthetase (HARS) gene is neurotoxic in vivo. · 2013 · https://pubmed.ncbi.nlm.nih.gov/22930593/ · DOI 10.1002/humu.22210","model_system":"Established enzymatic role in a primary HARS variant study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ce59e6c9-1213-523b-9d0b-400b7a81cd1c","stable_key":"import-911fb3c7-8cc3-5667-b677-5682fab67648","title":"L-Histidine: supply, catabolism, histamine, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"cb672530ec8b4a4542d0f3957e80ca76bb0449e9f988845b0f7681f623c4ec9b","revision_id":"19a7f26c-d9a1-5411-a81d-025f3d98a452","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}