{"id":"296aa3f8-530c-5a1b-9a52-dc62a2c308d4","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-trkb-activation","predicate":"increases_phosphorylation_of","statement":"Lithium increased TrkB Tyr490 phosphorylation after five days in rat cortical cultures.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f662508f-9370-5d83-9dc8-8141710c9b28","mechanism_event_label":"The BDNF receptor became more active after a delay.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"e7f904ee-c998-5ddd-911e-b8af8cb74404","slug":"rat-ntrk2","display_name":"Rat TrkB receptor / Ntrk2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f662508f-9370-5d83-9dc8-8141710c9b28","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-trkb-activation-event","event_type":"observed_relationship","label":"The BDNF receptor became more active after a delay.","description":"Lithium increased TrkB Tyr490 phosphorylation after five days in rat cortical cultures.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e7f904ee-c998-5ddd-911e-b8af8cb74404","slug":"rat-ntrk2","display_name":"Rat TrkB receptor / Ntrk2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8c87c2a6-f771-5d9e-b6ff-7b0f4923cd59","slug":"rat-bdnf","display_name":"Rat brain-derived neurotrophic factor / Bdnf","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat primary cortical neurons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pathway interpretation supported by inhibitor and antibody experiments; not direct lithium binding to TrkB.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The BDNF receptor became more active after a delay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lithium induces brain-derived neurotrophic factor and activates TrkB in rodent cortical neurons: an essential step for neuroprotection against glutamate excitotoxicity. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12504924/ · DOI 10.1016/s0028-3908(02)00217-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7790557f-eb96-5f5a-baed-b79a91e588e6","evidence_kind":"source_excerpt","locator":"Lines 216-222","start_line":216,"end_line":222,"excerpt":"## lithium-trkb-activation\nThe BDNF receptor became more active after a delay.\nLithium increased TrkB Tyr490 phosphorylation after five days in rat cortical cultures.\nModel: Rat primary cortical neurons.\nLimitations: Pathway interpretation supported by inhibitor and antibody experiments; not direct lithium binding to TrkB.\nEvidence access: Primary abstract\nLithium induces brain-derived neurotrophic factor and activates TrkB in rodent cortical neurons: an essential step for neuroprotection against glutamate excitotoxicity. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12504924/ · DOI 10.1016/s0028-3908(02)00217-4","model_system":"Rat primary cortical neurons.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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