{"id":"2950237d-93c1-5fc2-95ea-f22ed377c3aa","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-p5c-b6-trapping","predicate":"forms_adducts_with","statement":"P5C reacted with pyridoxal phosphate at pH 7.4 and 310 K to form three characterized adducts through condensation involving the PLP aldehyde group.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"db048681-2e35-5be4-bd30-df1b789bf401","mechanism_event_label":"An accumulating proline intermediate can chemically trap active vitamin B6.","subject":{"id":"cbf8b66e-3d66-53f0-9c21-bc9c014131e7","slug":"pyrroline-5-carboxylate","display_name":"Delta-1-pyrroline-5-carboxylate / P5C","entity_type_key":"small_molecule"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"db048681-2e35-5be4-bd30-df1b789bf401","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-p5c-b6-trapping-event","event_type":"observed_relationship","label":"An accumulating proline intermediate can chemically trap active vitamin B6.","description":"P5C reacted with pyridoxal phosphate at pH 7.4 and 310 K to form three characterized adducts through condensation involving the PLP aldehyde group.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cbf8b66e-3d66-53f0-9c21-bc9c014131e7","slug":"pyrroline-5-carboxylate","display_name":"Delta-1-pyrroline-5-carboxylate / P5C","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"12883df4-e1ea-548e-8d83-dd00b3bf377d","slug":"aldh4a1","display_name":"Human glutamate-5-semialdehyde dehydrogenase / ALDH4A1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free NMR, chromatography and mass-spectrometry experiments motivated by hyperprolinemia type II.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This demonstrates chemistry under the tested conditions, not B6 depletion from an ordinary proline-containing meal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An accumulating proline intermediate can chemically trap active vitamin B6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pyridoxal phosphate de-activation by pyrroline-5-carboxylic acid. Increased risk of vitamin B6 deficiency and seizures in hyperprolinemia type II. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11134058/ · DOI 10.1074/jbc.M010860200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"28e32e8e-dc76-58a3-977d-ca8041d4e283","evidence_kind":"source_excerpt","locator":"Lines 118-124","start_line":118,"end_line":124,"excerpt":"## l-proline-p5c-b6-trapping\nAn accumulating proline intermediate can chemically trap active vitamin B6.\nP5C reacted with pyridoxal phosphate at pH 7.4 and 310 K to form three characterized adducts through condensation involving the PLP aldehyde group.\nModel: Cell-free NMR, chromatography and mass-spectrometry experiments motivated by hyperprolinemia type II.\nLimitations: This demonstrates chemistry under the tested conditions, not B6 depletion from an ordinary proline-containing meal.\nEvidence access: Primary abstract\nPyridoxal phosphate de-activation by pyrroline-5-carboxylic acid. Increased risk of vitamin B6 deficiency and seizures in hyperprolinemia type II. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11134058/ · DOI 10.1074/jbc.M010860200","model_system":"Cell-free NMR, chromatography and mass-spectrometry experiments motivated by hyperprolinemia type II.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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