{"id":"2947df79-d29b-5765-a73f-decba772a93b","stable_key":"research:prdx3-reduces-mitochondrial-peroxide","predicate":"reduces","statement":"PRDX3 consumes hydrogen peroxide, forming water within the mitochondrial thioredoxin circuit.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:supported_interpretation","direction":"negative","is_public":true,"mechanism_event_id":"def4847e-cb10-51a9-ad45-517b92600ac0","mechanism_event_label":"The relay ends in peroxide removal.","subject":{"id":"ebb7ab23-2e3c-5334-8941-68f95c5879ea","slug":"prdx3","display_name":"PRDX3","entity_type_key":"protein"},"object":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"def4847e-cb10-51a9-ad45-517b92600ac0","stable_key":"research:prdx3-reduces-mitochondrial-peroxide","event_type":"peroxide-reduction","label":"The relay ends in peroxide removal.","description":"PRDX3 consumes hydrogen peroxide, forming water within the mitochondrial thioredoxin circuit.","status":"active","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"ebb7ab23-2e3c-5334-8941-68f95c5879ea","slug":"prdx3","display_name":"PRDX3","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"reduced to oxidized","sequence_order":0,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d31225b7-8e94-5857-9ea1-67ac794b1f15","slug":"water","display_name":"H2O","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"cardiac mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Peroxide and redox measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Other mitochondrial peroxide defenses also contribute.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse and guinea pig","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1d71e46b-d173-5c72-aaf7-45442d990c0f","evidence_kind":"curated_literature_summary","locator":"lines 630-640","start_line":630,"end_line":640,"excerpt":"## prdx3-reduces-mitochondrial-peroxide\n\nThe relay ends in peroxide removal.\n\nPRDX3 consumes hydrogen peroxide, forming water within the mitochondrial thioredoxin circuit.\n\nOrganism: mouse and guinea pig\nCell type: cardiac mitochondria\nExperimental model: Peroxide and redox measurements\nLimitations: Other mitochondrial peroxide defenses also contribute.\nPrimary reference: [Thioredoxin Reductase-2 Is Essential for Keeping Low Levels of H2O2 Emission from Isolated Heart Mitochondria](https://pubmed.ncbi.nlm.nih.gov/21832082/)","model_system":"Peroxide and redox measurements","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"mitochondrial-redox","plain_language":"The relay ends in peroxide removal.","evidence_scope":"supported_interpretation","papers":[{"key":"stanley-2011-txnrd2-relay","title":"Thioredoxin Reductase-2 Is Essential for Keeping Low Levels of H2O2 Emission from Isolated Heart Mitochondria","url":"https://pubmed.ncbi.nlm.nih.gov/21832082/","doi":"10.1074/jbc.M111.284612","year":2011,"model":"Mouse and guinea-pig cardiac mitochondria and cardiomyocytes","summary":"Tests the mitochondrial thioredoxin/peroxiredoxin peroxide-removal circuit using redox and functional measurements."}]}}