{"id":"2914662b-2d3d-543a-8aef-a27f08ce6abb","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-atp-product","predicate":"produces","statement":"Purified chicken carnosine synthase formed ADP stoichiometrically during carnosine synthesis with MgATP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3d5a6c13-12c1-5dff-bead-74fc011f788b","mechanism_event_label":"The synthesis reaction spends ATP and produces ADP.","subject":{"id":"8de50dc8-b6fb-525a-9e89-36b402e69054","slug":"chicken-carns1","display_name":"Chicken carnosine synthase / Carns1","entity_type_key":"protein"},"object":{"id":"136c3764-1b93-5f79-8673-9001cab9bc3d","slug":"adp","display_name":"Adenosine diphosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"3d5a6c13-12c1-5dff-bead-74fc011f788b","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-atp-product-event","event_type":"observed_relationship","label":"The synthesis reaction spends ATP and produces ADP.","description":"Purified chicken carnosine synthase formed ADP stoichiometrically during carnosine synthesis with MgATP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8de50dc8-b6fb-525a-9e89-36b402e69054","slug":"chicken-carns1","display_name":"Chicken carnosine synthase / Carns1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"136c3764-1b93-5f79-8673-9001cab9bc3d","slug":"adp","display_name":"Adenosine diphosphate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"288f19a6-62f0-51fe-8e6d-366388b6b16d","slug":"beta-alanine","display_name":"Beta-alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"24904faf-f4b4-5e5a-a2e6-3549fb7a2a2e","slug":"mg-atp","display_name":"Magnesium-ATP complex","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and full-text nucleotide analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chicken muscle enzyme and nucleotide analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Use ADP rather than the older inferred AMP assignment; no clinical magnesium-depletion threshold was measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The synthesis reaction spends ATP and produces ADP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Molecular identification of carnosine synthase as ATP-grasp domain-containing protein 1 (ATPGD1). · 2010 · https://pubmed.ncbi.nlm.nih.gov/20097752/ · DOI 10.1074/jbc.M109.095505","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"03a065e1-9e83-5019-8a8a-0fb0132a29dc","evidence_kind":"source_excerpt","locator":"Lines 28-34","start_line":28,"end_line":34,"excerpt":"## carnosine-atp-product\nThe synthesis reaction spends ATP and produces ADP.\nPurified chicken carnosine synthase formed ADP stoichiometrically during carnosine synthesis with MgATP.\nModel: Chicken muscle enzyme and nucleotide analysis.\nLimitations: Use ADP rather than the older inferred AMP assignment; no clinical magnesium-depletion threshold was measured.\nEvidence access: Primary abstract and full-text nucleotide analysis\nMolecular identification of carnosine synthase as ATP-grasp domain-containing protein 1 (ATPGD1). · 2010 · https://pubmed.ncbi.nlm.nih.gov/20097752/ · DOI 10.1074/jbc.M109.095505","model_system":"Chicken muscle enzyme and nucleotide analysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"87dc5af2-4eb6-5980-b7e0-bf3697b1156e","stable_key":"import-ae1ed35d-8ce7-5936-9067-a641374bbf79","title":"Carnosine: synthesis, transport, carbonyl chemistry and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"469cbc38203db56bd031f9d8a2dd2bfa387713950ca30199511cd137e927aa7a","revision_id":"cc409a15-8b04-50ee-a7ff-d1bf32f686bb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}