{"id":"28f6a33d-1dbd-5011-8f6d-0813aec641dc","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-hem25-glycine-rescue","predicate":"rescues_tested","statement":"Supplementing 5 mM glycine restored heme levels in yeast lacking Hem25 in the reported experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"045f6f01-db71-50b1-a6e5-27d7faac90d2","mechanism_event_label":"Extra substrate bypassed part of a transport bottleneck in yeast.","subject":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"object":{"id":"aedc209e-8cfa-5a81-925b-dcf8bf4e8736","slug":"yeast-hem25-heme","display_name":"Heme content in yeast lacking Hem25","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"045f6f01-db71-50b1-a6e5-27d7faac90d2","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-hem25-glycine-rescue-event","event_type":"observed_relationship","label":"Extra substrate bypassed part of a transport bottleneck in yeast.","description":"Supplementing 5 mM glycine restored heme levels in yeast lacking Hem25 in the reported experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"aedc209e-8cfa-5a81-925b-dcf8bf4e8736","slug":"yeast-hem25-heme","display_name":"Heme content in yeast lacking Hem25","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a965d3bd-c055-5ab9-99ee-29592aedb36a","slug":"yeast-hem25","display_name":"Yeast mitochondrial carrier Hem25","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Yeast hem25 deletion with glycine or 5-aminolevulinate supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Yeast has alternative pathways and synthesizes folate; this is not a human treatment result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Extra substrate bypassed part of a transport bottleneck in yeast.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glycine and Folate Ameliorate Models of Congenital Sideroblastic Anemia. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26821380/ · DOI 10.1371/journal.pgen.1005783","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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