{"id":"28c87c8f-43b2-5dad-a603-6ffb4ade2201","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-bovine-enos","predicate":"increases_tested_phosphorylation_of","statement":"SAC increased eNOS phosphorylation in BAE-1 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fa9f595a-8d43-5894-a912-20c471935998","mechanism_event_label":"A nitric-oxide-producing enzyme connects sulfur signaling to vascular biology.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"201a30a0-80c2-5a25-8be8-b75bc9df7164","slug":"bovine-nos3","display_name":"Bovine endothelial nitric oxide synthase / NOS3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fa9f595a-8d43-5894-a912-20c471935998","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-bovine-enos-event","event_type":"observed_relationship","label":"A nitric-oxide-producing enzyme connects sulfur signaling to vascular biology.","description":"SAC increased eNOS phosphorylation in BAE-1 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"201a30a0-80c2-5a25-8be8-b75bc9df7164","slug":"bovine-nos3","display_name":"Bovine endothelial nitric oxide synthase / NOS3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"Purified SAC and black garlic extract tested separately","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bovine aortic endothelial BAE-1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; 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phosphorylation site and human clinical effect not established by the accessed abstract.\nEvidence access: Primary abstract\n[37062967] Improving endothelial health with food-derived H2S donors: an in vitro study with S-allyl cysteine and with a black-garlic extract enriched in sulfur-containing compounds. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37062967/ · DOI 10.1039/d3fo00412k\nStructured context: {\"organism\": \"Bos taurus\", \"tissue_or_cell_type\": \"Bovine aortic endothelial BAE-1 cells\", \"dose\": \"Not specified in accessed abstract\", \"duration\": \"Not specified in accessed abstract\", \"route\": \"Cell culture\", \"experimental_comparison\": \"Purified SAC and black garlic extract tested separately\", \"acting_entity\": \"s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Bovine aortic endothelial BAE-1 cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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