{"id":"28c34a41-8607-5ebd-9567-f2185c397cbb","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-pon1-substrate","predicate":"hydrolyzes","statement":"Purified human PON1 hydrolyzed gamma-nonalactone at 144.7 +/- 11.3 micromol/min/mg.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"1087777c-0513-5373-8ce3-2b214791de9c","mechanism_event_label":"Purified human PON1 hydrolyzed gamma-nonalactone at 144.7 +/- 11.3 micromol/min/mg.","subject":{"id":"176ee7ea-3155-5966-a950-93cd29baa90d","slug":"pon1","display_name":"Human paraoxonase 1 / PON1","entity_type_key":"protein"},"object":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1087777c-0513-5373-8ce3-2b214791de9c","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-pon1-substrate-event","event_type":"observed_relationship","label":"Purified human PON1 hydrolyzed gamma-nonalactone at 144.7 +/- 11.3 micromol/min/mg.","description":"Purified human PON1 hydrolyzed gamma-nonalactone at 144.7 +/- 11.3 micromol/min/mg.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"176ee7ea-3155-5966-a950-93cd29baa90d","slug":"pon1","display_name":"Human paraoxonase 1 / PON1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Gamma-nonalactone 1 mM; Table 2 specific activities from 2-4 measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"PON2 non-detection tested up to 10 min at 37 C; exact positive-rate sampling interval not recovered","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary publisher Table 2, footnote and surrounding results plus PubMed abstract; complete assay methods not recovered.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant human PON1, PON2 and PON3, expressed in insect cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Gamma-nonalactone is a substrate, not a demonstrated PON activator. Buffer details were not recovered from the accessed table excerpt; physiological clearance and calcium-deficiency effects are unmeasured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Purified recombinant human PON1, PON2 and PON3, expressed in insect cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Purified human PON1 hydrolyzed gamma-nonalactone at 144.7 +/- 11.3 micromol/min/mg.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. (2005). https://pubmed.ncbi.nlm.nih.gov/15772423/ DOI: 10.1194/jlr.M400511-JLR200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro enzyme/substrate incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Cell-free lactone-hydrolysis assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2bc30bff-9641-562b-b479-6b766b120ca1","evidence_kind":"source_excerpt","locator":"Lines 103-112","start_line":103,"end_line":112,"excerpt":"## gamma-nonalactone-pon1-substrate\nPurified human PON1 hydrolyzed gamma-nonalactone at 144.7 +/- 11.3 micromol/min/mg.\nModel/species: Purified recombinant human PON1, PON2 and PON3, expressed in insect cells\nTissue: Cell-free lactone-hydrolysis assay\nExposure: Gamma-nonalactone 1 mM; Table 2 specific activities from 2-4 measurements\nRoute: In vitro enzyme/substrate incubation\nDuration: PON2 non-detection tested up to 10 min at 37 C; exact positive-rate sampling interval not recovered\nLimits: Gamma-nonalactone is a substrate, not a demonstrated PON activator. Buffer details were not recovered from the accessed table excerpt; physiological clearance and calcium-deficiency effects are unmeasured.\nPrimary reference: Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities. (2005). https://pubmed.ncbi.nlm.nih.gov/15772423/ DOI: 10.1194/jlr.M400511-JLR200\nAccess: Primary publisher Table 2, footnote and surrounding results plus PubMed abstract; complete assay methods not recovered.","model_system":"Purified recombinant human PON1, PON2 and PON3, expressed in insect cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73777c54-95a4-5b07-9d14-d4dd31949fb1","stable_key":"import-8600a864-6653-52c7-9718-1362c5af4577","title":"Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"a6fede6fbb2b70669be6846626f9c197edfd605c627d50fffa984e2b0b7ed71d","revision_id":"bb5ac9d2-d97d-59ac-a99c-ddf46d535574","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}