{"id":"283e58d0-5473-5251-a09e-2ef33d602460","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-glut3-dha","predicate":"transports","statement":"Mammalian GLUT3 expressed in Xenopus oocytes transported DHA with apparent Km 1.7 ± 0.3 mM; reduced ascorbate was not transported in the screen.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b1bed2d1-a1d5-5836-a489-269a3a4e72f3","mechanism_event_label":"GLUT3 can carry oxidized vitamin C; it does not substitute directly for an ascorbate transporter.","subject":{"id":"a8b00caa-810d-5cd5-94d7-13d9ad34c8a0","slug":"mammalian-glut3-orthologs","display_name":"Mammalian GLUT3 orthologs","entity_type_key":"protein_family"},"object":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b1bed2d1-a1d5-5836-a489-269a3a4e72f3","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-transport-glut3-dha-event","event_type":"biochemical_relationship","label":"GLUT3 can carry oxidized vitamin C; it does not substitute directly for an ascorbate transporter.","description":"Mammalian GLUT3 expressed in Xenopus oocytes transported DHA with apparent Km 1.7 ± 0.3 mM; reduced ascorbate was not transported in the screen.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"reduced-form comparison","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a8b00caa-810d-5cd5-94d7-13d9ad34c8a0","slug":"mammalian-glut3-orthologs","display_name":"Mammalian GLUT3 orthologs","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e6658c2b-ccff-5122-80a5-2e153f12ba90","slug":"dehydroascorbic-acid","display_name":"Dehydroascorbic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Heterologous GLUT expression and DHA uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In vitro apparent kinetics; donor species unresolved, so retained as ortholog group rather than a human-specific claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Xenopus laevis host; mammalian clone donor not verified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"GLUT3 can carry oxidized vitamin C; it does not substitute directly for an ascorbate transporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[rumsey1997] Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. (1997). https://pubmed.ncbi.nlm.nih.gov/9228080/ DOI: 10.1074/jbc.272.30.18982","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"DHA transport measured in expressing oocytes with an apparent Km of 1.7 mM.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"DHA transport measured in expressing oocytes with an apparent Km of 1.7 mM.","entity":null}],"evidence":[{"id":"dd7019d3-ad2b-5dcd-b957-c1d443949ae3","evidence_kind":"source_excerpt","locator":"Lines 260-271","start_line":260,"end_line":271,"excerpt":"### vc-transport-glut3-dha\nMammalian GLUT3 expressed in Xenopus oocytes transported DHA with apparent Km 1.7 ± 0.3 mM; reduced ascorbate was not transported in the screen.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: GLUT3 can carry oxidized vitamin C; it does not substitute directly for an ascorbate transporter.\norganism: Xenopus laevis host; mammalian clone donor not verified\ntissue_or_cell_type: Oocyte membrane\nexperimental_model: Mammalian GLUT cDNAs expressed in Xenopus laevis oocytes; CHO overexpression\nlimitations: In vitro apparent kinetics; donor species unresolved, so retained as ortholog group rather than a human-specific claim.\nexposure: Heterologous GLUT expression and DHA uptake\ncross_nutrient: false\n[rumsey1997] Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. 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