{"id":"28361a38-dbdf-51c6-9903-d049d58f6173","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cpt2-specificity","predicate":"discriminates_among","statement":"Human CPT2 had activity with C8-C18 acyl-CoAs but virtually none with short-chain acyl-CoAs or branched-chain amino-acid oxidation intermediates.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6d130464-e098-5e3a-a0a1-047d2ba44ce6","mechanism_event_label":"The long-chain shuttle is not the same enzyme system as short-chain buffering.","subject":{"id":"ba546852-649c-5632-a58d-702bc82bb6f5","slug":"human-cpt2","display_name":"Human carnitine palmitoyltransferase 2 / CPT2","entity_type_key":"protein"},"object":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6d130464-e098-5e3a-a0a1-047d2ba44ce6","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-cpt2-specificity-event","event_type":"observed_relationship","label":"The long-chain shuttle is not the same enzyme system as short-chain buffering.","description":"Human CPT2 had activity with C8-C18 acyl-CoAs but virtually none with short-chain acyl-CoAs or branched-chain amino-acid oxidation intermediates.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ba546852-649c-5632-a58d-702bc82bb6f5","slug":"human-cpt2","display_name":"Human carnitine palmitoyltransferase 2 / CPT2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a8093e02-6e69-592d-a171-f0821c802e28","slug":"propionylcarnitine","display_name":"Propionylcarnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human CPT2 substrate panel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No claim that every acylcarnitine originates from CPT2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The long-chain shuttle is not the same enzyme system as short-chain buffering.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Carnitine palmitoyltransferase 2: New insights on the substrate specificity and implications for acylcarnitine profiling. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20538056/ · DOI 10.1016/j.bbadis.2010.06.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7c8e43a9-ecdf-58a2-bbdd-389bca852ce0","evidence_kind":"source_excerpt","locator":"Lines 114-120","start_line":114,"end_line":120,"excerpt":"## l-carnitine-cpt2-specificity\nThe long-chain shuttle is not the same enzyme system as short-chain buffering.\nHuman CPT2 had activity with C8-C18 acyl-CoAs but virtually none with short-chain acyl-CoAs or branched-chain amino-acid oxidation intermediates.\nModel: Recombinant human CPT2 substrate panel.\nLimitations: No claim that every acylcarnitine originates from CPT2.\nEvidence access: Primary abstract\nCarnitine palmitoyltransferase 2: New insights on the substrate specificity and implications for acylcarnitine profiling. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20538056/ · DOI 10.1016/j.bbadis.2010.06.002","model_system":"Recombinant human CPT2 substrate panel.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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