{"id":"2829bed1-0016-5c5c-a488-edec41ab531b","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-peptide-helix","predicate":"promotes","statement":"Tartrazine favored the membrane-associated helical conformation of DHVAR4.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b22ba44a-49fc-56db-a7a0-c26132a30ece","mechanism_event_label":"Tartrazine favored the membrane-associated helical conformation of DHVAR4.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"184e3ed9-9948-507f-9670-32a674c7aab0","slug":"dhvar4-membrane-helical-conformation","display_name":"Membrane-associated helical conformation of DHVAR4","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b22ba44a-49fc-56db-a7a0-c26132a30ece","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-peptide-helix-event","event_type":"observed_relationship","label":"Tartrazine favored the membrane-associated helical conformation of DHVAR4.","description":"Tartrazine favored the membrane-associated helical conformation of DHVAR4.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"184e3ed9-9948-507f-9670-32a674c7aab0","slug":"dhvar4-membrane-helical-conformation","display_name":"Membrane-associated helical conformation of DHVAR4","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b894725c-45b0-57aa-a5ae-f0de7cc5fbf0","slug":"dhvar4","display_name":"DHVAR4 antimicrobial peptide","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"DHVAR4 plus tartrazine; concentrations and ratios not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Assay intervals not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract; unrecovered method details explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No clinical antimicrobial efficacy or effect on all natural histatins is established. Species-specific bactericidal thresholds and mammalian exposure require the full protocol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine favored the membrane-associated helical conformation of DHVAR4.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anionic food color tartrazine enhances antibacterial efficacy of histatin-derived peptide DHVAR4 by fine-tuning its membrane activity. (2020). https://pubmed.ncbi.nlm.nih.gov/32115014/ DOI: 10.1017/S0033583520000013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro mixing and membrane/bacterial assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Peptide-dye association and membrane activity","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5b6d22b1-3466-5ab7-b681-9bcc2b0fed0c","evidence_kind":"source_excerpt","locator":"Lines 413-422","start_line":413,"end_line":422,"excerpt":"## tartrazine-peptide-helix\nTartrazine favored the membrane-associated helical conformation of DHVAR4.\nModel/species: Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures\nTissue: Peptide-dye association and membrane activity\nExposure: DHVAR4 plus tartrazine; concentrations and ratios not specified in accessed abstract\nRoute: In vitro mixing and membrane/bacterial assays\nDuration: Assay intervals not specified in accessed abstract\nLimits: No clinical antimicrobial efficacy or effect on all natural histatins is established. Species-specific bactericidal thresholds and mammalian exposure require the full protocol.\nPrimary reference: Anionic food color tartrazine enhances antibacterial efficacy of histatin-derived peptide DHVAR4 by fine-tuning its membrane activity. (2020). https://pubmed.ncbi.nlm.nih.gov/32115014/ DOI: 10.1017/S0033583520000013\nAccess: Primary PubMed abstract; unrecovered method details explicitly retained.","model_system":"Synthetic histatin-derived DHVAR4 peptide, model lipid bilayers and tested bacterial cultures","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. 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