{"id":"2816f201-5f9a-5cef-b814-7f5725d6786e","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-rac1","predicate":"reduces_tested_activation_of","statement":"Cucurbitacin I reduced heregulin-evoked Rac1 activation in human breast-cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ea49504a-0fc5-51b0-a8b3-8a1c0dc74522","mechanism_event_label":"A movement-control switch became less active.","subject":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"object":{"id":"8a96d7c7-d28d-5615-94a7-3c397bed8f9d","slug":"rac1","display_name":"RAC1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ea49504a-0fc5-51b0-a8b3-8a1c0dc74522","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-rac1-event","event_type":"observed_relationship","label":"A movement-control switch became less active.","description":"Cucurbitacin I reduced heregulin-evoked Rac1 activation in human breast-cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8a96d7c7-d28d-5615-94a7-3c397bed8f9d","slug":"rac1","display_name":"RAC1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a1bf9392-212b-513c-a985-d7775cd2e9a0","slug":"human-breast-cui-motility","display_name":"Human breast-cancer-cell motility during cucurbitacin I exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human breast-cancer cell experiments; representative 0.1 micromolar exposure for 1 hour.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Activity change is not direct Rac1 binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A movement-control switch became less active.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin I inhibits Rac1 activation in breast cancer cells by a reactive oxygen species-mediated mechanism and independently of Janus tyrosine kinase 2 and P-Rex1. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23478800/ · DOI 10.1124/mol.112.084293","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c5e9bf63-c775-5ae3-b267-77ec4b9749cc","evidence_kind":"source_excerpt","locator":"Lines 156-162","start_line":156,"end_line":162,"excerpt":"## cucurbitacin-i-rac1\nA movement-control switch became less active.\nCucurbitacin I reduced heregulin-evoked Rac1 activation in human breast-cancer cells.\nModel: Human breast-cancer cell experiments; representative 0.1 micromolar exposure for 1 hour.\nLimitations: Activity change is not direct Rac1 binding.\nEvidence access: Primary abstract\nCucurbitacin I inhibits Rac1 activation in breast cancer cells by a reactive oxygen species-mediated mechanism and independently of Janus tyrosine kinase 2 and P-Rex1. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23478800/ · DOI 10.1124/mol.112.084293","model_system":"Human breast-cancer cell experiments; representative 0.1 micromolar exposure for 1 hour.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}