{"id":"28079142-a61e-5cce-84c2-a08116ee7654","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-cyp-adduct","predicate":"produces","statement":"Mass spectra indicated cinnamaldehyde-associated CYP2A6 apoprotein adduction, with reported mass addition about 132.67 Da; added glutathione did not prevent this measured adduction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d548340f-fc62-5a10-8064-7e516f297df8","mechanism_event_label":"The protein acquired extra mass even with glutathione present. This differs from measuring how much enzyme activity survives.","subject":{"id":"2dc093c6-9373-5b24-a17b-a6b21b5a0432","slug":"trans-cinnamaldehyde","display_name":"trans-Cinnamaldehyde / cinnamal","entity_type_key":"small_molecule"},"object":{"id":"bdc00b2b-f371-5592-93e7-6cb43c996dc3","slug":"cyp2a6-apoprotein-adduction","display_name":"Cinnamaldehyde-associated CYP2A6 apoprotein mass adduction","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d548340f-fc62-5a10-8064-7e516f297df8","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-cyp-adduct-event","event_type":"biochemical_relationship","label":"The protein acquired extra mass even with glutathione present. 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Heme loss was specifically reported for the methoxy analogue; apoprotein adduction is a different endpoint from inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ceylon","display_name":"Ceylon cinnamon / Cinnamomum verum bark preparations","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human CYP2A6; rat-scaled modeling parameters","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The protein acquired extra mass even with glutathione present. This differs from measuring how much enzyme activity survives.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ceylon-p32788161] Mechanisms of Herb-Drug Interactions Involving Cinnamon and CYP2A6: Focus on Time-Dependent Inhibition by Cinnamaldehyde and 2-Methoxycinnamaldehyde. (2020). https://pubmed.ncbi.nlm.nih.gov/32788161/ DOI: 10.1124/dmd.120.000087","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Enzyme apoprotein/heme; simulated drug exposure","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2cfcc8e6-6d37-5829-a5e6-b61f62723103","evidence_kind":"source_excerpt","locator":"Lines 766-777","start_line":766,"end_line":777,"excerpt":"### ceylon-cyp-adduct\nMass spectra indicated cinnamaldehyde-associated CYP2A6 apoprotein adduction, with reported mass addition about 132.67 Da; added glutathione did not prevent this measured adduction.\nCondition category: normal\nnutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The protein acquired extra mass even with glutathione present. This differs from measuring how much enzyme activity survives.\norganism: Human CYP2A6; rat-scaled modeling parameters\ntissue_or_cell_type: Enzyme apoprotein/heme; simulated drug exposure\nexperimental_model: CYP2A6 mass spectrometry, time-dependent inhibition and numerical modeling\nlimitations: Rat-scaled predicted human AUC is not observed AUC. Heme loss was specifically reported for the methoxy analogue; apoprotein adduction is a different endpoint from inhibition.\nexposure: Cinnamaldehyde and separately 2-methoxycinnamaldehyde; model exposure approximately 275 mg cinnamaldehyde\nevidence_span: {\"source_cache\": \"artifacts/ceylon-research/32788161.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"431169f83241cb83b84dd81d84eb1db225735677cabf33f09af8b1e22dfe64f4\", \"start_char\": 0, \"end_char\": 2507, \"text_sha256\": \"431169f83241cb83b84dd81d84eb1db225735677cabf33f09af8b1e22dfe64f4\"}\n[ceylon-p32788161] Mechanisms of Herb-Drug Interactions Involving Cinnamon and CYP2A6: Focus on Time-Dependent Inhibition by Cinnamaldehyde and 2-Methoxycinnamaldehyde. (2020). https://pubmed.ncbi.nlm.nih.gov/32788161/ DOI: 10.1124/dmd.120.000087","model_system":"CYP2A6 mass spectrometry, time-dependent inhibition and numerical modeling","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ceylon-p32788161] Mechanisms of Herb-Drug Interactions Involving Cinnamon and CYP2A6: Focus on Time-Dependent Inhibition by Cinnamaldehyde and 2-Methoxycinnamaldehyde. (2020). https://pubmed.ncbi.nlm.nih.gov/32788161/ DOI: 10.1124/dmd.120.000087","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3aed3c5b-8016-5e37-ad6f-9019d34d19eb","stable_key":"import-3d41d18a-e13a-59a0-a081-55bfcdd43e55","title":"Ceylon cinnamon: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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