{"id":"27d2acb6-7dfc-5527-bc1d-95a29cd583d2","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-does-not-change-pth-biosynthesis-in-slice-assay","predicate":"did-not-alter","statement":"Mg concentration did not measurably change PTH biosynthesis or proPTH-to-PTH conversion in the bovine slice experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"9e3eb23b-77e6-5c7b-8615-1466d948b31b","mechanism_event_label":"Mg changed release of the hormone without showing the same effect on making or processing it in this assay.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"8d3df0fb-7e38-5399-a832-2967f6e7ac9d","slug":"pth-biosynthesis","display_name":"Parathyroid hormone biosynthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9e3eb23b-77e6-5c7b-8615-1466d948b31b","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-does-not-change-pth-biosynthesis-in-slice-assay-event","event_type":"biochemical_relationship","label":"Mg changed release of the hormone without showing the same effect on making or processing it in this assay.","description":"Mg concentration did not measurably change PTH biosynthesis or proPTH-to-PTH conversion in the bovine slice experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e162b0ab-1449-5471-b3c3-4638a0fa3a96","slug":"pth","display_name":"Parathyroid hormone / PTH","entity_type_key":"protein"},"role":"hormone product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8d3df0fb-7e38-5399-a832-2967f6e7ac9d","slug":"pth-biosynthesis","display_name":"Parathyroid hormone biosynthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"magnesium -> PTH -> calcium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiolabeled leucine incorporation and precursor/hormone analysis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A negative result in this exposure range does not exclude synthesis defects during prolonged severe deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Bos taurus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mg changed release of the hormone without showing the same effect on making or processing it in this assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[habener-1976-pth-synthesis-secretion] Relative effectiveness of magnesium and calcium on the secretion and biosynthesis of parathyroid hormone in vitro (1976). https://pubmed.ncbi.nlm.nih.gov/1248443/ DOI: 10.1210/endo-98-1-197","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Parathyroid gland","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6028a927-d0ed-5956-9d87-5bbc506a561d","evidence_kind":"source_excerpt","locator":"Lines 265-275","start_line":265,"end_line":275,"excerpt":"### mg-does-not-change-pth-biosynthesis-in-slice-assay\nMg concentration did not measurably change PTH biosynthesis or proPTH-to-PTH conversion in the bovine slice experiments.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Mg changed release of the hormone without showing the same effect on making or processing it in this assay.\norganism: Bos taurus\ntissue_or_cell_type: Parathyroid gland\nexperimental_model: Radiolabeled leucine incorporation and precursor/hormone analysis\nlimitations: A negative result in this exposure range does not exclude synthesis defects during prolonged severe deficiency.\ncross_nutrient: magnesium -> PTH -> calcium\n[habener-1976-pth-synthesis-secretion] Relative effectiveness of magnesium and calcium on the secretion and biosynthesis of parathyroid hormone in vitro (1976). https://pubmed.ncbi.nlm.nih.gov/1248443/ DOI: 10.1210/endo-98-1-197","model_system":"Radiolabeled leucine incorporation and precursor/hormone analysis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [habener-1976-pth-synthesis-secretion] Relative effectiveness of magnesium and calcium on the secretion and biosynthesis of parathyroid hormone in vitro (1976). https://pubmed.ncbi.nlm.nih.gov/1248443/ DOI: 10.1210/endo-98-1-197","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}