{"id":"2790d2a9-f345-5898-839f-ae1def9582ac","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-mate1","predicate":"transports","statement":"Human MATE1 expression increased saturable agmatine accumulation, with Km 240 ± 31 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"62b5b206-9cc4-597c-8ea0-75d897e10d52","mechanism_event_label":"A transporter associated with renal secretion recognizes agmatine.","subject":{"id":"198b7bfb-7e85-5c91-94b7-32603452a8e3","slug":"slc47a1","display_name":"Human multidrug and toxin extrusion protein 1 / MATE1 / SLC47A1","entity_type_key":"protein"},"object":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"62b5b206-9cc4-597c-8ea0-75d897e10d52","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-mate1-event","event_type":"observed_relationship","label":"A transporter associated with renal secretion recognizes agmatine.","description":"Human MATE1 expression increased saturable agmatine accumulation, with Km 240 ± 31 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"198b7bfb-7e85-5c91-94b7-32603452a8e3","slug":"slc47a1","display_name":"Human multidrug and toxin extrusion protein 1 / MATE1 / SLC47A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human MATE1 in HEK293 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Accumulation direction in the assay is not identical to net secretion across an intact kidney.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A transporter associated with renal secretion recognizes agmatine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"OCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Expression increased saturable agmatine accumulation in the cell.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Expression increased saturable agmatine accumulation in the cell.","entity":null}],"evidence":[{"id":"c01e9639-c7e3-5c4e-848b-01538dd8dd9e","evidence_kind":"source_excerpt","locator":"Lines 36-42","start_line":36,"end_line":42,"excerpt":"## agmatine-sulfate-mate1\nA transporter associated with renal secretion recognizes agmatine.\nHuman MATE1 expression increased saturable agmatine accumulation, with Km 240 ± 31 micromolar.\nModel: Human MATE1 in HEK293 cells.\nLimitations: Accumulation direction in the assay is not identical to net secretion across an intact kidney.\nEvidence access: Primary abstract\nOCT2 and MATE1 provide bidirectional agmatine transport. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21128598/ · DOI 10.1021/mp100180a","model_system":"Human MATE1 in HEK293 cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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