{"id":"274c74f1-3a3b-5d61-9090-02dd7cd7e304","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:coa-import-acetyl-null","predicate":"reported_relationship","statement":"Isolated K562 mitochondria showed negligible uptake of acetyl-labeled acetyl-CoA under the tested assay.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"2ea998ad-aada-51ae-bbeb-2d292c57c6aa","mechanism_event_label":"Isolated K562 mitochondria showed negligible uptake of acetyl-labeled acetyl-CoA under the tested assay.","subject":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"object":{"id":"7c0c8467-4c75-5dc1-8b8f-c66f587dbca6","slug":"human-isolated-mito-acetylcoa-uptake","display_name":"Human isolated-mitochondrial acetyl-CoA uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2ea998ad-aada-51ae-bbeb-2d292c57c6aa","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:coa-import-acetyl-null-event","event_type":"experimental_observation","label":"Isolated K562 mitochondria showed negligible uptake of acetyl-labeled acetyl-CoA under the tested assay.","description":"### coa-import-acetyl-null\n\nIsolated K562 mitochondria showed negligible uptake of acetyl-labeled acetyl-CoA under the tested assay.\nCoA-backbone labeling after free CoASH uptake must not be mistaken for direct import of intact acetyl-CoA. A small residual signal could reflect membrane binding or minimal import.\nModel and exposure: Fig. 7d–e; 10 micromolar 13C2-acetyl-CoA, 6 min, 20–25 C.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism. | 2025 | https://pubmed.ncbi.nlm.nih.gov/40925986/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC12460162/ | https://doi.org/10.1038/s42255-025-01358-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"7c0c8467-4c75-5dc1-8b8f-c66f587dbca6","slug":"human-isolated-mito-acetylcoa-uptake","display_name":"Human isolated-mitochondrial acetyl-CoA uptake","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Fig. 7d–e; 10 micromolar 13C2-acetyl-CoA, 6 min, 20–25 C.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Isolated K562 mitochondria showed negligible uptake of acetyl-labeled acetyl-CoA under the tested assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism. | https://pubmed.ncbi.nlm.nih.gov/40925986/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC12460162/ | https://doi.org/10.1038/s42255-025-01358-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference coa-import-acetyl-null; CoA-backbone labeling after free CoASH uptake must not be mistaken for direct import of intact acetyl-CoA. A small residual signal could reflect membrane binding or minimal import.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"737c1018-d1bb-5724-a060-704bb2534b7a","evidence_kind":"source_excerpt","locator":"Lines 76-82","start_line":76,"end_line":82,"excerpt":"### coa-import-acetyl-null\n\nIsolated K562 mitochondria showed negligible uptake of acetyl-labeled acetyl-CoA under the tested assay.\nCoA-backbone labeling after free CoASH uptake must not be mistaken for direct import of intact acetyl-CoA. A small residual signal could reflect membrane binding or minimal import.\nModel and exposure: Fig. 7d–e; 10 micromolar 13C2-acetyl-CoA, 6 min, 20–25 C.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Cellular pan-chain acyl-CoA profiling reveals SLC25A42/SLC25A16 in mitochondrial CoA import and metabolism. | 2025 | https://pubmed.ncbi.nlm.nih.gov/40925986/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC12460162/ | https://doi.org/10.1038/s42255-025-01358-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Fig. 7d–e; 10 micromolar 13C2-acetyl-CoA, 6 min, 20–25 C.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}