{"id":"2749e491-e223-5dd3-955e-8068ce440385","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-mouse-lipt2-octanoyl-transfer","predicate":"catalyzes","statement":"Purified mouse Lipt2 transferred octanoyl groups from E. coli ACP to human GCSH.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4ce14e5b-1421-514d-8823-6f281788ee03","mechanism_event_label":"The first assembly step loads an eight-carbon precursor onto GCSH.","subject":{"id":"9b7a74af-1478-557f-8423-636ae03727b2","slug":"mouse-lipt2","display_name":"Mouse octanoyltransferase / Lipt2","entity_type_key":"protein"},"object":{"id":"05891ff0-fe54-5c5d-9ca2-5fe3c39bf603","slug":"gcsh-octanoyl","display_name":"Human octanoyl-GCSH","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"4ce14e5b-1421-514d-8823-6f281788ee03","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-mouse-lipt2-octanoyl-transfer-event","event_type":"biochemical_relationship","label":"The first assembly step loads an eight-carbon precursor onto GCSH.","description":"Purified mouse Lipt2 transferred octanoyl groups from E. coli ACP to human GCSH.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1d8aefc7-8b5e-50ed-baa3-063be4f53b7c","slug":"octanoyl-acp","display_name":"Octanoyl acyl-carrier protein","entity_type_key":"protein_state"},"role":"donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6238d82b-cb8d-5477-ab46-db40608715eb","slug":"e-coli-acpp","display_name":"Escherichia coli acyl carrier protein / AcpP","entity_type_key":"protein"},"role":"donor_protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"78a93278-7e43-59c7-b097-31bbae21c771","slug":"gcsh","display_name":"Human glycine-cleavage H-protein / GCSH","entity_type_key":"protein"},"role":"acceptor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9b7a74af-1478-557f-8423-636ae03727b2","slug":"mouse-lipt2","display_name":"Mouse octanoyltransferase / Lipt2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"05891ff0-fe54-5c5d-9ca2-5fe3c39bf603","slug":"gcsh-octanoyl","display_name":"Human octanoyl-GCSH","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/29987032.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d0569075b59128ea021be6077596127d1c7aae1f9ac6b0acfd2ebcb268b650d3\", \"start_char\": 16562, \"end_char\": 20381, \"text_sha256\": \"34c5cb398fa99661566112ccf850c1fe0e4a5e74e3aa5b355b1695d12cb7b24d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified enzymes and bacterial pathway reconstruction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Recombinant proteins and radiolabeled substrates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human and mouse constructs must not be conflated. Recombinant reconstruction establishes chemistry, not supplementation efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human proteins; mouse Lipt2 in purified transfer assays; E. coli host","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The first assembly step loads an eight-carbon precursor onto GCSH.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p29987032] Protein moonlighting elucidates the essential human pathway catalyzing lipoic acid assembly on its cognate enzymes. (2018). https://pubmed.ncbi.nlm.nih.gov/29987032/ DOI: 10.1073/pnas.1805862115","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Lipoyl assembly pathway","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d5dea0d3-9e3b-5118-bcff-d26ef5d7f7b3","evidence_kind":"source_excerpt","locator":"Lines 221-232","start_line":221,"end_line":232,"excerpt":"### ala-mouse-lipt2-octanoyl-transfer\nPurified mouse Lipt2 transferred octanoyl groups from E. coli ACP to human GCSH.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The first assembly step loads an eight-carbon precursor onto GCSH.\norganism: Human proteins; mouse Lipt2 in purified transfer assays; E. coli host\ntissue_or_cell_type: Lipoyl assembly pathway\nexperimental_model: Purified enzymes and bacterial pathway reconstruction\nlimitations: Human and mouse constructs must not be conflated. Recombinant reconstruction establishes chemistry, not supplementation efficacy.\nexposure: Recombinant proteins and radiolabeled substrates\nevidence_span: {\"source_cache\": \"artifacts/ala-research/29987032.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d0569075b59128ea021be6077596127d1c7aae1f9ac6b0acfd2ebcb268b650d3\", \"start_char\": 16562, \"end_char\": 20381, \"text_sha256\": \"34c5cb398fa99661566112ccf850c1fe0e4a5e74e3aa5b355b1695d12cb7b24d\"}\n[ala-p29987032] Protein moonlighting elucidates the essential human pathway catalyzing lipoic acid assembly on its cognate enzymes. (2018). https://pubmed.ncbi.nlm.nih.gov/29987032/ DOI: 10.1073/pnas.1805862115","model_system":"Purified enzymes and bacterial pathway reconstruction","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ala-p29987032] Protein moonlighting elucidates the essential human pathway catalyzing lipoic acid assembly on its cognate enzymes. (2018). https://pubmed.ncbi.nlm.nih.gov/29987032/ DOI: 10.1073/pnas.1805862115","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d8afa8c2-ced9-5b28-90ca-2ac120ec7202","stable_key":"import-5d8e27d8-6a74-5560-827f-3f90908bbc34","title":"Alpha-lipoic acid: cofactor assembly, redox signaling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"611da198ab85a272eb26b64ee330ef6d1a762853a8481f3191c4acfca9f3cf3d","revision_id":"618b4ce6-3157-5222-8325-408064be5ea5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}