{"id":"2744217f-1e98-53c2-90f6-31fd229a8c4a","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-pparg-binding","predicate":"binds","statement":"Surface-plasmon-resonance assays detected binding of astaxanthin to recombinant human PPAR-gamma ligand-binding domains.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b0209b3d-85fe-5495-94e9-afa53a55c1f4","mechanism_event_label":"Direct binding and the direction of transcriptional effects are separate questions.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b0209b3d-85fe-5495-94e9-afa53a55c1f4","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-pparg-binding-event","event_type":"observed_relationship","label":"Direct binding and the direction of transcriptional effects are separate questions.","description":"Surface-plasmon-resonance assays detected binding of astaxanthin to recombinant human PPAR-gamma ligand-binding domains.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, methods 2.4 and SPR results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human receptor-domain biochemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reporter effects depend on construct, cell and coactivator context.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Direct binding and the direction of transcriptional effects are separate questions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The natural carotenoid astaxanthin, a PPAR-α agonist and PPAR-γ antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22707263/ · DOI 10.1002/mnfr.201100798","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e3a86fbb-ff00-5dca-a6ab-72afdd540cb7","evidence_kind":"source_excerpt","locator":"Lines 254-260","start_line":254,"end_line":260,"excerpt":"## astaxanthin-pparg-binding\nDirect binding and the direction of transcriptional effects are separate questions.\nSurface-plasmon-resonance assays detected binding of astaxanthin to recombinant human PPAR-gamma ligand-binding domains.\nModel: Recombinant human receptor-domain biochemistry.\nLimitations: Reporter effects depend on construct, cell and coactivator context.\nEvidence access: Primary full text, methods 2.4 and SPR results\nThe natural carotenoid astaxanthin, a PPAR-α agonist and PPAR-γ antagonist, reduces hepatic lipid accumulation by rewiring the transcriptome in lipid-loaded hepatocytes. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22707263/ · DOI 10.1002/mnfr.201100798","model_system":"Recombinant human receptor-domain biochemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4fd91ea6-78b4-5bfe-93e3-686fbef9f678","stable_key":"import-a8bedee0-a740-5bbb-921e-bfd144c7b68c","title":"Astaxanthin: transport, membrane chemistry, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"98640e32f62e6c0387578165342ad21b3d2325701fcfdbb5c48886c783040e5e","revision_id":"a82757ce-1feb-582c-a5fc-f7de36308f1f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}