{"id":"273b420b-dae7-5899-8ac2-d888da7c1ad8","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-collagen-production","predicate":"increases","statement":"Orthosilicic acid at 10–20 micromolar increased type I collagen synthesis in human osteoblast-like cultures.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"77494df9-36d3-5ee0-8ae6-6302cede80bf","mechanism_event_label":"Bone-forming cells produced more collagen scaffold.","subject":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"object":{"id":"091a1ec0-5867-5659-b199-b73a1ac8b9c6","slug":"human-type-i-collagen","display_name":"Human type I collagen","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"77494df9-36d3-5ee0-8ae6-6302cede80bf","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-collagen-production-event","event_type":"observed_relationship","label":"Bone-forming cells produced more collagen scaffold.","description":"Orthosilicic acid at 10–20 micromolar increased type I collagen synthesis in human osteoblast-like cultures.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"091a1ec0-5867-5659-b199-b73a1ac8b9c6","slug":"human-type-i-collagen","display_name":"Human type I collagen","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"MG-63, HCC1 and primary human marrow-derived osteoblast-like cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture synthesis is not demonstrated human bone strength or fracture benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Bone-forming cells produced more collagen scaffold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12633784/ · DOI 10.1016/s8756-3282(02)00950-x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"678291a8-3836-58dc-a165-37795e224e5a","evidence_kind":"source_excerpt","locator":"Lines 120-126","start_line":120,"end_line":126,"excerpt":"## silica-collagen-production\nBone-forming cells produced more collagen scaffold.\nOrthosilicic acid at 10–20 micromolar increased type I collagen synthesis in human osteoblast-like cultures.\nModel: MG-63, HCC1 and primary human marrow-derived osteoblast-like cells.\nLimitations: Cell-culture synthesis is not demonstrated human bone strength or fracture benefit.\nEvidence access: Primary abstract\nOrthosilicic acid stimulates collagen type 1 synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro. · 2003 · https://pubmed.ncbi.nlm.nih.gov/12633784/ · DOI 10.1016/s8756-3282(02)00950-x","model_system":"MG-63, HCC1 and primary human marrow-derived osteoblast-like cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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