{"id":"273adffe-2260-5ee6-8286-27ac15dc8d8f","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-vglut3-chloride","predicate":"activates","statement":"Purified human VGLUT3 also showed chloride-dependent glutamate transport in reconstituted vesicles.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"848b6a19-e762-5e6b-b8b2-9af7d2d6b061","mechanism_event_label":"A human transporter isoform shares a tested regulatory input.","subject":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"object":{"id":"d2308a43-8681-5261-8d59-4a3e1c4d999e","slug":"slc17a8","display_name":"Human vesicular glutamate transporter 3 / SLC17A8","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"848b6a19-e762-5e6b-b8b2-9af7d2d6b061","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-vglut3-chloride-event","event_type":"observed_relationship","label":"A human transporter isoform shares a tested regulatory input.","description":"Purified human VGLUT3 also showed chloride-dependent glutamate transport in reconstituted vesicles.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d2308a43-8681-5261-8d59-4a3e1c4d999e","slug":"slc17a8","display_name":"Human vesicular glutamate transporter 3 / SLC17A8","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human VGLUT3 expressed in insect cells and reconstituted separately from rat VGLUT2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Isoforms and species are kept distinct; no nutritional chloride threshold was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A human transporter isoform shares a tested regulatory input.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic control of vesicular glutamate transport and release. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20920794/ · DOI 10.1016/j.neuron.2010.09.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"edf328c5-22fa-5ddf-aafe-04e8f58b41a5","evidence_kind":"source_excerpt","locator":"Lines 290-296","start_line":290,"end_line":296,"excerpt":"## glutamate-vglut3-chloride\nA human transporter isoform shares a tested regulatory input.\nPurified human VGLUT3 also showed chloride-dependent glutamate transport in reconstituted vesicles.\nModel: Human VGLUT3 expressed in insect cells and reconstituted separately from rat VGLUT2.\nLimitations: Isoforms and species are kept distinct; no nutritional chloride threshold was tested.\nEvidence access: Primary full text\nMetabolic control of vesicular glutamate transport and release. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20920794/ · DOI 10.1016/j.neuron.2010.09.002","model_system":"Human VGLUT3 expressed in insect cells and reconstituted separately from rat VGLUT2.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}