{"id":"27103267-0314-54fe-a27d-f7975f98d516","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip5-basolateral","predicate":"localizes_to","statement":"Mouse ZIP5 localized to the basolateral membrane when expressed in polarized MDCK cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"87f8d125-3af0-5d59-a4f5-9c980fa47864","mechanism_event_label":"In polarized kidney-derived cells, ZIP5 sat on the side facing the tissue rather than the lumen.","subject":{"id":"5d71153b-6474-5bc7-89ff-666b940683da","slug":"mouse-slc39a5","display_name":"Mouse ZIP5 (Slc39a5)","entity_type_key":"protein"},"object":{"id":"38e0912b-83f6-5dde-880f-7b0c279f4f73","slug":"polarized-cell-basolateral-membrane","display_name":"Polarized epithelial basolateral membrane","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"87f8d125-3af0-5d59-a4f5-9c980fa47864","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip5-basolateral-event","event_type":"biochemical_relationship","label":"In polarized kidney-derived cells, ZIP5 sat on the side facing the tissue rather than the lumen.","description":"Mouse ZIP5 localized to the basolateral membrane when expressed in polarized MDCK cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"transporter_cargo_context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5d71153b-6474-5bc7-89ff-666b940683da","slug":"mouse-slc39a5","display_name":"Mouse ZIP5 (Slc39a5)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38e0912b-83f6-5dde-880f-7b0c279f4f73","slug":"polarized-cell-basolateral-membrane","display_name":"Polarized epithelial basolateral membrane","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Polarized Madin-Darby canine kidney cells expressing mouse ZIP5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Polarized-cell localization assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Localization supports a directional hypothesis but does not itself measure net intestinal zinc excretion or demonstrate human renal zinc reabsorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse protein in Canis lupus familiaris cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In polarized kidney-derived cells, ZIP5 sat on the side facing the tissue rather than the lumen.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-15322118] The mammalian Zip5 protein is a zinc transporter that localizes to the basolateral surface of polarized cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15322118/ DOI: 10.1074/jbc.m408361200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"MDCK epithelial basolateral membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"04290d4f-aed8-5647-8fe5-a87e46a2dfa5","evidence_kind":"source_excerpt","locator":"Lines 375-386","start_line":375,"end_line":386,"excerpt":"### zinc-trans-zip5-basolateral\nMouse ZIP5 localized to the basolateral membrane when expressed in polarized MDCK cells.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In polarized kidney-derived cells, ZIP5 sat on the side facing the tissue rather than the lumen.\norganism: Mouse protein in Canis lupus familiaris cells\ntissue_or_cell_type: MDCK epithelial basolateral membrane\nexperimental_model: Polarized Madin-Darby canine kidney cells expressing mouse ZIP5\nlimitations: Localization supports a directional hypothesis but does not itself measure net intestinal zinc excretion or demonstrate human renal zinc reabsorption.\nexposure: Polarized-cell localization assay.\ncross_nutrient: false\n[zinc-trans-15322118] The mammalian Zip5 protein is a zinc transporter that localizes to the basolateral surface of polarized cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15322118/ DOI: 10.1074/jbc.m408361200","model_system":"Polarized Madin-Darby canine kidney cells expressing mouse ZIP5","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-15322118] The mammalian Zip5 protein is a zinc transporter that localizes to the basolateral surface of polarized cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15322118/ DOI: 10.1074/jbc.m408361200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}