{"id":"27055486-e658-5054-be3a-ca2efae02fa2","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-mlc2-junction","predicate":"reduces","statement":"Sodium butyrate reduced MLC2 Ser19 phosphorylation during Caco-2 tight-junction reassembly.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b3ac6456-b9a4-5077-9817-a572627317f9","mechanism_event_label":"The contractile machinery at the cell boundary changed as junctions reformed.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"ae55ed03-4f4d-5353-a79f-e4c9c3d66f57","slug":"human-butyrate-mlc2-phosphorylation","display_name":"MLC2 Ser19 phosphorylation in butyrate-exposed Caco-2 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b3ac6456-b9a4-5077-9817-a572627317f9","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-mlc2-junction-event","event_type":"observed_relationship","label":"The contractile machinery at the cell boundary changed as junctions reformed.","description":"Sodium butyrate reduced MLC2 Ser19 phosphorylation during Caco-2 tight-junction reassembly.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ae55ed03-4f4d-5353-a79f-e4c9c3d66f57","slug":"human-butyrate-mlc2-phosphorylation","display_name":"MLC2 Ser19 phosphorylation in butyrate-exposed Caco-2 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bf38462d-0c44-58c8-a619-befacd80fe54","slug":"human-butyrate-tight-junction-assembly","display_name":"Human Caco-2 tight-junction assembly after butyrate","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 phosphorylation measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"MLC2 assay identity is retained without guessing the gene isoform from its shorthand name.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The contractile machinery at the cell boundary changed as junctions reformed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27735862/ · DOI 10.3390/ijms17101696","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"55b95afc-2b07-5727-af45-5a8f534220f9","evidence_kind":"source_excerpt","locator":"Lines 286-292","start_line":286,"end_line":292,"excerpt":"## butyrate-mlc2-junction\nThe contractile machinery at the cell boundary changed as junctions reformed.\nSodium butyrate reduced MLC2 Ser19 phosphorylation during Caco-2 tight-junction reassembly.\nModel: Human Caco-2 phosphorylation measurements.\nLimitations: MLC2 assay identity is retained without guessing the gene isoform from its shorthand name.\nEvidence access: Primary abstract\nSodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27735862/ · DOI 10.3390/ijms17101696","model_system":"Human Caco-2 phosphorylation measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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