{"id":"26fa9423-32a5-512d-a6fe-4134c16de000","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-rat-scd1-response","predicate":"reduces_tested","statement":"Methionine restriction reduced hepatic Scd1 expression and serum fatty-acid desaturation indices; cysteine supplementation reversed these changes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e660e35f-03ce-5e7c-9a5e-0677ec965e26","mechanism_event_label":"Sulfur-amino-acid composition influenced a lipid-metabolism enzyme.","subject":{"id":"2fa490e6-50cc-5a22-b1ea-af5e128666e4","slug":"experimental-methionine-restriction","display_name":"Experimentally methionine-restricted diet","entity_type_key":"cellular_process"},"object":{"id":"96c936e1-0140-51e3-83d3-e1440701108c","slug":"rat-scd1","display_name":"Rat stearoyl-CoA desaturase 1 / Scd1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e660e35f-03ce-5e7c-9a5e-0677ec965e26","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-rat-scd1-response-event","event_type":"observed_relationship","label":"Sulfur-amino-acid composition influenced a lipid-metabolism enzyme.","description":"Methionine restriction reduced hepatic Scd1 expression and serum fatty-acid desaturation indices; cysteine supplementation reversed these changes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2fa490e6-50cc-5a22-b1ea-af5e128666e4","slug":"experimental-methionine-restriction","display_name":"Experimentally methionine-restricted diet","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"96c936e1-0140-51e3-83d3-e1440701108c","slug":"rat-scd1","display_name":"Rat stearoyl-CoA desaturase 1 / Scd1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat liver RNA/protein and serum lipid profiling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Desaturation indices are indirect activity readouts; no isolated human enzyme effect is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Sulfur-amino-acid composition influenced a lipid-metabolism enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cysteine supplementation reverses methionine restriction effects on rat adiposity: significance of stearoyl-coenzyme A desaturase. · 2011 · https://pubmed.ncbi.nlm.nih.gov/20871132/ · DOI 10.1194/jlr.M010215","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f29de53e-c206-5e05-b033-66bdad1be837","evidence_kind":"source_excerpt","locator":"Lines 372-378","start_line":372,"end_line":378,"excerpt":"## methionine-rat-scd1-response\nSulfur-amino-acid composition influenced a lipid-metabolism enzyme.\nMethionine restriction reduced hepatic Scd1 expression and serum fatty-acid desaturation indices; cysteine supplementation reversed these changes.\nModel: Rat liver RNA/protein and serum lipid profiling.\nLimitations: Desaturation indices are indirect activity readouts; no isolated human enzyme effect is established.\nEvidence access: Primary abstract\nCysteine supplementation reverses methionine restriction effects on rat adiposity: significance of stearoyl-coenzyme A desaturase. · 2011 · https://pubmed.ncbi.nlm.nih.gov/20871132/ · DOI 10.1194/jlr.M010215","model_system":"Rat liver RNA/protein and serum lipid profiling.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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