{"id":"26f96176-b9e8-564f-ae2d-9534141d923e","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-tkt-radiolabeled-thdp-binding","predicate":"binds","statement":"Sulfur-35-labeled ThDP binding was measured directly in human erythrocyte transketolase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7256957a-f69e-598c-b9e7-48fb047e4e0c","mechanism_event_label":"Tracer binding measures the vitamin-derived cofactor joining the enzyme.","subject":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"object":{"id":"c95c645c-ae67-5911-871e-4f796a17faec","slug":"tkt","display_name":"Human transketolase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7256957a-f69e-598c-b9e7-48fb047e4e0c","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-tkt-radiolabeled-thdp-binding-event","event_type":"biochemical_relationship","label":"Tracer binding measures the vitamin-derived cofactor joining the enzyme.","description":"Sulfur-35-labeled ThDP binding was measured directly in human erythrocyte transketolase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"native enzyme-associated metal","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c95c645c-ae67-5911-871e-4f796a17faec","slug":"tkt","display_name":"Human transketolase","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human erythrocyte transketolase; sulfur-35 ThDP binding and divalent-ion reconstitution.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding measurement alone does not establish tissue flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tracer binding measures the vitamin-derived cofactor joining the enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[jung-1988-tkt] Studies on the nature of thiamine pyrophosphate binding and dependency on divalent cations of transketolase from human erythrocytes (1988). https://pubmed.ncbi.nlm.nih.gov/3248678/ DOI: 10.1016/0020-711x(88)90228-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Erythrocyte enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5d385e39-2f05-5879-b3e9-6e902ed0393a","evidence_kind":"source_excerpt","locator":"Lines 541-551","start_line":541,"end_line":551,"excerpt":"### mg-tkt-radiolabeled-thdp-binding\nSulfur-35-labeled ThDP binding was measured directly in human erythrocyte transketolase.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Tracer binding measures the vitamin-derived cofactor joining the enzyme.\norganism: Homo sapiens\ntissue_or_cell_type: Erythrocyte enzyme\nexperimental_model: Human erythrocyte transketolase; sulfur-35 ThDP binding and divalent-ion reconstitution.\nlimitations: Binding measurement alone does not establish tissue flux.\ncross_nutrient: Magnesium availability supports vitamin B1 activation or cofactor use in the specified preparation; this does not establish a dietary threshold or universal treatment failure.\n[jung-1988-tkt] Studies on the nature of thiamine pyrophosphate binding and dependency on divalent cations of transketolase from human erythrocytes (1988). https://pubmed.ncbi.nlm.nih.gov/3248678/ DOI: 10.1016/0020-711x(88)90228-5","model_system":"Human erythrocyte transketolase; sulfur-35 ThDP binding and divalent-ion reconstitution.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [jung-1988-tkt] Studies on the nature of thiamine pyrophosphate binding and dependency on divalent cations of transketolase from human erythrocytes (1988). https://pubmed.ncbi.nlm.nih.gov/3248678/ DOI: 10.1016/0020-711x(88)90228-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}