{"id":"26e15c37-0d85-5724-b590-96cd38f84210","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-microfilaria-direct-kill-null","predicate":"no_detected_effect_on","statement":"Ivermectin had little direct effect on Brugia malayi microfilariae in culture at pharmacologically relevant concentrations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"43b07b79-9008-5e86-bc2b-df60f5aa1616","mechanism_event_label":"Ivermectin had little direct effect on Brugia malayi microfilariae in culture at pharmacologically relevant concentrations.","subject":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"object":{"id":"35059d4f-9a4c-5b3e-91aa-3d176da2b107","slug":"brugia-microfilaria-viability","display_name":"Brugia malayi microfilarial viability in culture","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"43b07b79-9008-5e86-bc2b-df60f5aa1616","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-microfilaria-direct-kill-null-event","event_type":"observed_relationship","label":"Ivermectin had little direct effect on Brugia malayi microfilariae in culture at pharmacologically relevant concentrations.","description":"Ivermectin had little direct effect on Brugia malayi microfilariae in culture at pharmacologically relevant concentrations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"35059d4f-9a4c-5b3e-91aa-3d176da2b107","slug":"brugia-microfilaria-viability","display_name":"Brugia malayi microfilarial viability in culture","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Brugia malayi microfilariae in culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Pharmacologically relevant ivermectin concentrations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A null result in culture does not exclude killing in the host, where immune clearance is available; it is the reason the secretion mechanism was investigated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Brugia malayi microfilariae in culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Ivermectin had little direct effect on Brugia malayi microfilariae in culture at pharmacologically relevant concentrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Ivermectin disrupts the function of the excretory-secretory apparatus in microfilariae of Brugia malayi. (2010). https://pubmed.ncbi.nlm.nih.gov/21041637/ DOI: 10.1073/pnas.1011983107","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Parasite viability","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"089c28dc-87f1-5a96-98e0-ae7a1f7dded5","evidence_kind":"source_excerpt","locator":"Lines 46-55","start_line":46,"end_line":55,"excerpt":"## ivermectin-microfilaria-direct-kill-null\nIvermectin had little direct effect on Brugia malayi microfilariae in culture at pharmacologically relevant concentrations.\nModel/species: Brugia malayi microfilariae in culture\nTissue/system: Parasite viability\nExposure: Pharmacologically relevant ivermectin concentrations\nRoute: In vitro\nDuration: Not stated here\nLimits: A null result in culture does not exclude killing in the host, where immune clearance is available; it is the reason the secretion mechanism was investigated.\nPrimary reference: Ivermectin disrupts the function of the excretory-secretory apparatus in microfilariae of Brugia malayi. (2010). https://pubmed.ncbi.nlm.nih.gov/21041637/ DOI: 10.1073/pnas.1011983107\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"20818ddd-7b37-5226-a24b-7de007e80eb2","stable_key":"import-e0801c92-7fd3-5cb2-82b7-dd82f010f3ed","title":"Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"c7e1e0201cbd222f0c52b7981f078936ed39b617d747821ed6daa584c2b964a7","revision_id":"56746c3e-cdec-5b8f-a191-425de1a0a7ce","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}